中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 3T3-L1前脂肪细胞中Ghrelin对miRNAs表达的影响 动物医学进展 2020 (7) 68-73
Position: Home > Articles > Effect of Ghrelin on miRNAs Expression in 3T3-L1 Preadipocytes Progress in Veterinary Medicine 2020 (7) 68-73

3T3-L1前脂肪细胞中Ghrelin对miRNAs表达的影响

作  者：
郭东光;陈明艳;陈健;朱艳平;崔芳微;李文明;孙丽莎;李玉谷
单  位：
关键词：
Ghrelin;miRNA;3T3-L1前脂肪细胞;真核表达质粒
摘  要：
为探讨3T3-L1前脂肪细胞增殖分化过程中Ghrelin对microRNAs(miRNAs)表达的影响,将pcDNA3.1(+)-Ghrelin转染至3T3-L1前脂肪细胞,real-time PCR检测10种miRNA的表达变化,并对显著差异表达的miRNA进行生物信息学分析。结果显示,pcDNA3.1(+)-Ghrelin的表达明显上调,约是对照组的5倍;real-time PCR结果显示,miR-125b-5p(P<0.05)表达显著上调,miR-21a-5p(P<0.01)、miR-30c-5p(P<0.05)、miR-181-5p(P<0.01)表达显著下调;生物信息学分析结果显示,预测调节的1 062个靶基因与细胞的生长、分化和发育功能相关,核因子1b(Nfib)是其共同调节的重要潜在靶基因;丝裂原活化蛋白激酶(MAPK signaling pathway)和成脂基因信号通路是其调节差异最为显著的信号通路,与脂肪细胞的增殖和分化密切相关。结果表明,Ghrelin可通过调节miRNA的表达影响3T3-L1前脂肪细胞的增殖和分化。研究结果为进一步探讨Ghrelin发挥效应的分子调节机制和分子调控网络奠定了基础。
译  名：
Effect of Ghrelin on miRNAs Expression in 3T3-L1 Preadipocytes
作  者：
GUO Dong-guang;CHEN Ming-yan;CHEN Jian;ZHU Yan-ping;CUI Fang-wei;LI Wen-ming;SUN Li-sha;LI Yu-gu;Bio-technology Centre/School of Life Science,Xinxiang University;College of Veterinary Medicine,South China Agricultural University;Xinxiang People's Park;
关键词：
Ghrelin;;microRNAs;;3T3-L1 cell;;eukaryotic expression plasmid
摘  要：
To investigate the effect of ghrelin on the expression of microRNAs(miRNAs)during the proliferation and differentiation of 3 T3-L1 preadipocytes,pcDNA3.1(+)-Ghrelin was transfected into 3 T3-L1 preadipocytes,and the expressions of 10 miRNAs were detected by qPCR,the differentially expressed miRNAs were analyzed by bioinformatics.The results showed that the expression of pcDNA3.1(+)-Ghrelin was significantly up-regulated,about five times higher than that of the control group; the qPCR results showed that the expression of miR-125 b-5 p(P<0.05) was significantly up-regulated,and the expression of miR-21 a-5 p(P<0.01),miR-30 c-5 p(P<0.05) and miR-181-5 p(P<0.01) were significantly down-regulated; the bioinformatics analysis showed that the 1062 target genes predicted for regulation were related to the cell growth,differentiation and developmental functions,and nuclear factor 1 b-type(Nfib) was a potential important target gene for its co-regulation.Both the MAPK and adipogenesis gene pathways are the most significant signaling pathways that regulated by miRNAs,which are closely related to adipocyte proliferation and differentiation.These results suggest that Ghrelin can affect the proliferation and differentiation of 3 T3-L1 preadipocytes by regulating the expression of miRNAs.This study laid a foundation for further exploring the molecular regulatory mechanism and network of Ghrelin effect.

相似文章

导出引用
计量
文章访问数: 11
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46