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野生毛百合与松叶百合组培快繁技术研究

作  者:
汪娜;郭太君;李雪;陈少鹏
单  位:
吉林农业大学园艺学院;吉林省林业科学院
关键词:
毛百合;松叶百合;组织培养
摘  要:
以毛百合与松叶百合不同部位的鳞片为外植体,研究了不同消毒方式、不同激素组合和不同部位鳞片对毛百合和松叶百合不定芽诱导和增殖的影响.结果表明:毛百合用75%酒精30s+30%84消毒液15min消毒效果最好;鳞茎的最佳诱导培养基为MS+0.5mg/L 6-BA+0.5mg/L NAA;最佳不定芽增殖培养基MS+0.3mg/L 6-BA+0.2mg/L NAA.松叶百合鳞茎的最佳诱导培养基为MS+1.0mg/L 6-BA+0.3mg/L NAA;芽的增殖培养基为MS+1.0mg/L6-BA+0.1mg/L 2,4-D.外植体取样部位以鳞茎中层鳞片的基部较好.
单  位:
College of Horticulture,Jilin Agricultural University,Changchun,Jilin 130118%Jilin Academy of Forestry Sciences,Jilin,Jilin 132013
关键词:
Lilium dauricum%Lilium cernum%tissue culture
摘  要:
Taking different parts of squama of the bulb scales of Lilium dauricum and L.cernum as explants,the effect of different sterilize method and different hormones composing and different parts of a squama on their adventitious bud induction and multiplication were studied.The results showed the bulb scales treated with 75% alcohol for 30 s followed by 30% 84 disinfectant for 15 min exhibited the best sterilization effect;the suitable medium for bulb induction of L.dauricum was MS+6-BA 0.5 mg/L+NAA 0.5 mg/L;the optimum medium for adventitious bud proliferation was MS+6-BA 0.3 mg/L+NAA 0.2 mg/L.The suitable medium for bulb induction of L.cernum was MS+6-BA 1.0 mg/L+NAA 0.3 mg/L,MS+6-BA 1.0 mg/L+2,4-D 0.1 mg/L was better for adventitious bud proliferation.The better sampling sites of explants were the base of the bulbs central scales.

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