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Position: Home > Articles > PCR-SSCP Analysis on AHCY Gene in Holstein Cows China Animal Husbandry & Veterinary Medicine 2009,36 (11) 95-98

荷斯坦母牛AHCY基因PCR-SSCP分析

作  者:
李拓;储明星;王金玉;安永福;狄冉;方丽
单  位:
河北省畜牧兽医研究所;扬州大学动物科学与技术学院;中国农业科学院北京畜牧兽医研究所
关键词:
荷斯坦母牛;AHCY基因;PCR-SSCP
摘  要:
根据已发表的牛AHCY基因序列设计13对引物,采用PCR-SSCP方法在210头荷斯坦母牛中检测S-腺苷高半胱氨酸水解酶(S-adenosylhomocysteine hydrolase,AHCY)基因全部10个外显子的单核苷酸多态性,同时分析该基因对荷斯坦母牛乳房炎抗性的影响。结果表明,AHCY基因的10个外显子在检测的荷斯坦母牛中都不存在多态性,AHCY基因外显子区域可能不存在影响乳房炎抗性的遗传标记。
译  名:
PCR-SSCP Analysis on AHCY Gene in Holstein Cows
作  者:
LI Tuo1,CHU Ming-xing2,WANG Jin-yu1,AN Yong-fu3,DI Ran2,FANG Li2(1.College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China;2.Key Laboratory of Farm Animal Genetic Resources and Utilization of Ministry of Agriculture, Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China;3.Hebei Animal Science and Veterinary Medicine Institute,Baoding 071000,China)
关键词:
Holstein cows;AHCY gene;PCR-SSCP
摘  要:
The AHCY(S-adenosylhomocysteine hydrolase) gene was studied as a candidate gene for mastitis resistance in 210 Holstein cows.Single nucleotide polymorphisms(SNPs) of all exons(exons 1 to 10) of AHCY gene were detected with thirteen pairs of primers by polymerase chain reaction-single strand conformation polymorphism(PCR-SSCP) in 210 Holstein cows.No polymorphism was detected in the amplified region in the ten exons tested,this result indicated that the genetic markers that affect the mastitis resistance in Holstein cows may not exist in the detected exons of AHCY gene.

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