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当前位置: 首页 > 文章 > 牦牛和黄牛三个微卫星基因座种间特异性等位基因分析 甘肃农业大学学报 2010,45 (4) 22-27
Position: Home > Articles > Species specific alleles at three microsatellite loci in yak and cattle Journal of Gansu Agricultural University 2010,45 (4) 22-27

牦牛和黄牛三个微卫星基因座种间特异性等位基因分析

作  者：
冯冬梅;赵会静;罗玉柱;韩建林
单  位：
甘肃农业大学动物科学技术学院;中国农业科学院北京畜牧兽医研究所
关键词：
牦牛;黄牛;微卫星DNA标记;物种特异性等位基因
摘  要：
采用PCR产物直接测序和克隆测序相结合的方法分析了牦牛、普通牛和瘤牛在ILSTS013、ILSTS050和SPS115 3个微卫星基因座上存在的特异性等位基因座位的特异性.结果表明:牦牛、普通牛和瘤牛在这3个基因座上等位基因的DNA序列存在种间特异性差异,依据这些特点可以将牦牛与普通牛和瘤牛在基因型或DNA序列水平上进行区分,这将成为评价牛种间的基因交流水平和建立牛肉及其产品分子追溯系统的可靠分子标记.
译  名：
Species specific alleles at three microsatellite loci in yak and cattle
作  者：
FENG Dong-mei1,2,ZHAO Hui-jing2,3,LUO Yu-zhu1,HAN Jian-lin2,3(1.College of Animal Science and Technology,Gansu Agricultural University,Lanzhou 730073,China;2.Key Laboratory of Farm Animal Genetic Resources and Utilization of Ministry of Agriculture,Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193,China;3.CAAS-ILRI Joint Laboratory on Livestock and Forage Genetic Resources,Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193,China)
关键词：
yak;cattle;microsatellite DNA marker;species specific allele
摘  要：
The species specific alleles at three microsatellite DNA loci of ILSTS013,ILSTS050 and SPS115 were verified using direct PCR product sequencing and/or cloning sequencing methods.The results confirmed that yak,taurine and indicine cattle had different sequence characteristics to each other in the alleles at these three loci,leading to species specific allele sizes in genotyping exercise.Therefore these three loci can be used for discriminating alleles originated from yak,taurine and indicine cattle in assessment of the level of genetic admixture between bovine species and in establishment of molecular traceability systems for beef and its products from different bovine species.

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