当前位置: 首页 > 文章 > 基于ITS标记分析安徽省桑种质资源的亲缘关系 热带农业工程 2017,41 (2) 31-35
Position: Home > Articles > An Analysis on Genetic Relationship of Mulberry Germplasm Resources Collected in Anhui Province Based on ITS Marker Tropical Agricultural Engineering 2017,41 (2) 31-35

基于ITS标记分析安徽省桑种质资源的亲缘关系

作  者:
王钰婷;李瑞雪;孙帆;王伟;汪泰初
单  位:
安徽省农业科学院蚕桑研究所
关键词:
桑树种质资源;ITS序列;进化分析
摘  要:
核糖体DNA内转录间隔区(internal transcribed spacer,ITS)是被子植物分子系统发育研究中应用最广泛的分子标记。对收集的12份安徽地方特色桑种质资源提取总DNA,用PCR扩增方法进行测序分析;根据鲁桑基因序列,确定ITS序列范围,并进行序列比对;通过分析序列长度、G+C含量、变异位点及遗传分歧,探讨了安徽桑树种质材料间的亲缘关系,为更深入地研究桑属植物间的系统进化关系提供重要的参考资料。
译  名:
An Analysis on Genetic Relationship of Mulberry Germplasm Resources Collected in Anhui Province Based on ITS Marker
作  者:
WANG Yuting;LI Ruixue;SUN Fan;WANG Wei;WANG Taichu;Sericultural Research Institute,Anhui Academy of Agricultural Science;
单  位:
Sericultural Research Institute,Anhui Academy of Agricultural Science
关键词:
Mulberry germplasm resources;;ITS marker;;phylogeny
摘  要:
Ribosome transcribed spacer(ITS)is the most widely used nuclear DNA sequence in molecular systems.The sequencing analysis was carried out by using the total DNA extraction and PCR amplification method.According to the sequence of Morus multicaulis gene,the ITS sequence range was determined and sequence alignment was performed.By analyzing the sequence length,G+ C content and mutation loci and genetic divergence,the phylogenetic relationship between the collected mulberry germplasm in Anhui Province was discussed,which provided the basic reference for the further study of phylogenetic evolution between mulberry plants.

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