中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 三黄鸡临床血管瘤病例中分离出A亚群与J亚群禽白血病病毒 动物医学进展 2015 (3) 13-16
Position: Home > Articles > Isolation of Subgroup A and J ALV in the Clinical Hemangioma of Three-Yellow Chicken Progress in Veterinary Medicine 2015 (3) 13-16

三黄鸡临床血管瘤病例中分离出A亚群与J亚群禽白血病病毒

作  者：
王培坤;毕玉彧;秦丽莉;邹广珍;彭昊;韦平
单  位：
广西大学养禽与禽病学研究所
关键词：
共感染;A亚群禽白血病病毒;J亚群禽白血病病毒;gp85;聚合酶链反应
摘  要：
为了解禽白血病病毒在广西鸡群中流行情况,采用DF-1细胞接种、细胞培养上清p27抗原检测、PCR扩增,对临床血管瘤型禽白血病的三黄鸡病料进行了病毒分离,并对分离病毒gp85基因进行测序和序列比较。结果表明,从1只病鸡同时分离到了一株A亚群禽白血病病毒(ALV-A)与一株J亚群禽白血病病毒(ALV-J),分别命名为HG01-A株和HG01-J株。ALV-A gp85与7株A亚群氨基酸同源性为85.8%~87.5%,与A亚群美国株MQNCSU同源性最高为87.5%,与A亚群原型株RSA同源性为86.9%。而ALV-J gp85与7株毒株核酸序列同源性为84.0%~93.8%,与广东株XX2-08以及四川株SCSM01同源性最高为93.8%,与英国原型株HPRS103同源性为90.2%。进化分析进一步表明,HG01-A与各参考株亲缘关系较远,HG01-J与SCSM01亲缘关系最近。本研究首次从同一只广西三黄鸡中同时分离到ALV-A及ALV-J,进一步完善了我国地方品种鸡群中禽白血病的流行病学信息。
译  名：
Isolation of Subgroup A and J ALV in the Clinical Hemangioma of Three-Yellow Chicken
作  者：
WANG Pei-kun;BI Yu-yu;QIN Li-li;ZOU Guang-zhen;PENG Hao;WEI Ping;Institute for Poultry Science and Health,Guangxi University;
关键词：
co-infection;;ALV-J;;ALV-A;;gp85;;PCR
摘  要：
In order to understand the avian leukosis virus epidemic situation in Guangxi chickens,two strains of avian leukosis virus subgroup A and J were isolated in Three-yellow chickens in the cultured DF-1cells,which were identified by ALV p27 detection and PCR amplification and sequence analysis of avian leukosis virus gp85 gene,and then were named as HG01-A and HG01-J,respectively.The results of variation analysis of gp85 gene indicated that the homology between HG01-A and 7reference strains was85.8%-87.5%,and shared the highest homology with MQNCSU at 87.5%,shared the homology with RSA at 86.9%.The results of variation analysis of gp85 gene indicated that the homology between HG01-J and 7reference strains was 84.0%-93.8%,and shared the highest homology withXX2-08 and SCSM01at92.8%,shared the homology withHPRS1-3at 90.2%.The present results indicated that it the first report that there was the co-infection of ALV-A and ALV-J in the Guangxi Three-yellow chickens,and provided new epidemiological information of avian leucosis virus in local chicken.

相似文章

导出引用
计量
文章访问数: 7
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46