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Position: Home > Articles > Effects of Cr~(3+) on soil enzyme activity Journal of Northwest A & F University(Natural Science Edition) 2012,40 (5) 166-172

Cr~(3+)的土壤酶效应研究

作  者:
胡一;孔龙;和文祥;吕家珑
单  位:
中国科学院/水利部水土保持研究所;西北农林科技大学资源环境学院
关键词:
Cr3+;土壤酶;生态剂量;作用机理;总体酶活性
摘  要:
【目的】研究Cr3+对土壤酶活性的影响,为建立土壤重金属铬污染评价的酶学指标提供依据。【方法】以我国常见的红壤(酸性土壤)及褐土和风沙土(碱性土壤)为供试土样,采用室内模拟方法,在供试土样中添加0,50,100,300,500,1 000,1 500,3 000mg/kg Cr3+,研究不同Cr3+含量下土壤脲酶、磷酸酶、芳基硫酸酯酶、过氧化氢酶、脱氢酶和总体酶活性的变化规律。【结果】Cr3+显著或极显著地抑制了土壤脲酶、磷酸酶、芳基硫酸酯酶、过氧化氢酶和脱氢酶活性,且均为完全抑制作用(包括竞争性抑制和非竞争性抑制),供试土样中Cr3+轻度污染时的生态剂量(ED10)为25mg/kg;酸性土壤中脲酶、芳基硫酸酯酶和过氧化氢酶对Cr3+的毒害反应更敏感,碱性土壤中磷酸酶和脱氢酶的反应更敏感。【结论】土壤脲酶、磷酸酶、芳基硫酸酯酶、过氧化氢酶、脱氢酶活性及总体酶活性均可用来表征土壤Cr3+污染程度的大小;土壤pH、有机质含量对土壤酶与Cr3+的关系具有重要影响。
译  名:
Effects of Cr~(3+) on soil enzyme activity
作  者:
HU Yia,KONG Longb,HE Wen-xianga,Lü Jia-longa(a College of Resources and Environment,b College of Life Sciences,Northwest A&F University,Yangling,Shaanxi 712100,China)
关键词:
Cr3+;soil enzymes;ecological dose;mechanism;total enzyme activity
摘  要:
【Objective】 Influence of Cr3+ on soil enzymes was studied to provide a foundation for enzyme assessment index in soils polluted by heavy metals.【Method】 Red soil,Cinnamonl and sandy soil,main soil types in China,were sampled.By simulative method,soil urease,phosohatase,arylsulphatase,catalase,dehydrogenase and total enzyme activities were studied under different Cr3+ concentrations 0,50,100,300,500,1 000,1 500,3 000 mg/kg.【Result】 Soil urease,phosohatase,arylsulphatase,catalase,dehydrogenases enzymes significant or remarkably significant negative correlation with Cr3+ concentrations was obtained.And the reaction mechanism was complete inhibition(including competitive inhibition and noncompetitive inhibition).The critical Cr3+ concentration was 25 mg/kg when the soils were slightly polluted(ED10),arylsufatase and catalase were more sensitive to Cr3+ in acid soils,however,in alkalinen soil phosohatase and dehydrogenases were more sensitive.【Conclusion】 Soil urease,phosohatase,arylsulphatase,catalase,dehydrogenases enzymes and total enzyme activity could be index to monitor soils polluted by Cr3+,soil enzyme impact was up to soil physic-chemical properties,such as pH and organic matter content.

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