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Acta Agronomica Sinica
1998,24
(4)
410-415
烟草叶绿体转化载体的构建及转基因植株的获得
作 者：
邹竹荣;张中林;山松;倪丕冲;沈桂芳
单 位：
中国农业科学院生物技术研究中心
关键词：
叶绿体基因组同源片段;叶绿体转化;aadA;基因枪轰击
摘 要：
选择烟草叶绿体基因组同源片段rp12-trnH-psbA和trnK-ORF509A,及aadA抗壮观霉素基因,构建烟草叶绿体转化载体pTRZ。制备pTRZ DNA金粉子弹,通过基因枪轰击烟草幼苗叶片,经壮观霉素筛选获得了愈伤组织和转化再生植株。对烟草叶绿体转化植株进行PCR和Southern分析,结果证明其中No.13、16、23为整入了外源aadA的叶绿体转基因植株,同时其子代呈现壮观霉素抗性,aadA基因得到稳定遗传。
译 名：
Construction of Vector for Tobacco Plastid Transformation and Obtainment of Tobacco Transgenic Plants
作 者：
Zou Zhurong Zhang Zhonglin Shan Song Ni Pichong Shen Guifang
(Biotechnology Research Center, Chinese Academy of Agricultural Sciences, Beijing, 100081)
关键词：
Plastid homologous recombinant fragment; Plastid transformation; aadA; Microprojectiles bombardment
摘 要：
Vector pTRZ was constructed with rpl2-trnH-psbA, trnK-ORF509A as to
bacco plastid homologous recombinant fragments and spectinomycin-resistant aadA gene from
plasmid pZS197. After spectinomycin selection, transformed calli and regenerated tobacco plants
were obtained from tobacco seedling's leaves bombarded with golden microprojectiles coated with
pTRZ DNA. Further PCR and Southern analyses of transformed plants indicated that No. 13,
16, 23 were positive tobacco transplastomic plants and the foreign aadA gene had integrated into
their plastid genome. The resistance of progeny phenotype to spectinomycin showed the aadA
gene was stably inherited to their progeny.
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