当前位置: 首页 > 文章 > 北美红杉组织培养技术研究 西部林业科学 2010,39 (4) 48-51
Position: Home > Articles > Studies on the Tissue Culture of Sequoia sempervirens Journal of West China Forestry Science 2010,39 (4) 48-51

北美红杉组织培养技术研究

作  者:
周元超;徐凌彦;于进英;李枝林
单  位:
云南农业大学园林园艺学院;云南省农业职业技术学院
关键词:
北美红杉;组织培养;继代培养;生根
摘  要:
通过不同激素对北美红杉离体繁殖影响的实验,建立了相应的组培技术体系。研究结果表明,北美红杉茎段外植体经消毒后接种在MS+IBA 0.1 mg/L+KT 1.0 mg/L上诱导,出芽率最高;北美红杉继代增殖随着继代次数的增加,逐步降低细胞分裂素6-BA的浓度,而获得健壮的植株;生根培养以1/4MS+NAA 1.5 mg/L+IBA 2.5 mg/L培养基效果最好,生根率为74%,且枯顶率和萌蘖率也较低。
译  名:
Studies on the Tissue Culture of Sequoia sempervirens
作  者:
ZHOU Yuan-chao1,XU Ling-yan2,YU Jin-ying1,LI Zhi-lin1(1.College of Horticulture and Landscape,Yunnan Agricultural University,Kunming Yunnan 650201,P.R.China;2.Yunnan Agricultural Science and Technology College,Kunming Yunnan 650031,P.R.China)
关键词:
Sequoia sempervirens;tissue culture;subculture;rooting
摘  要:
The tissue culture technology system of Sequoia sempervirens was established using the introduced plant materials and studying the effects of different hormones.The results indicated that putting the sterilized stem sections on MS+IBA 0.1 mg/L+KT 1.0 mg/L could get the highest germination rate.In the procedure of subculture,the concentrations of adding 6-BA should be decreased gradually for getting healthy plant.The optimum medium for rooting was 1/4MS+NAA 1.5 mg/L+IBA 2.5 mg/L,with the rooting rate of 74 %,moreover the rate of wilt top and sprouting were all low.

相似文章

计量
文章访问数: 11
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊