Position: Home > Articles > Optimization Separation of Three Adrenalines and Quantitative Analysis in Portulaca oleracea L. by Capillary Zone Electrophoresis
Journal of Anhui Agricultural Sciences
2012,40
(32)
15601-15602+15607
CZE法用于肾上腺素的优化分离及其在马齿苋中含量测定的研究
作 者：
高苏亚;王黎;李华;李静;孙文基
单 位：
西北大学陕西省生物医药重点实验室;西北大学分析科学研究所;西安医学院药学院
关键词：
区带毛细管电泳;肾上腺素;马齿苋;优化分离;测定
摘 要：
[目的]采用区带毛细管电泳法实现去氧肾上腺素、肾上腺素和去甲肾上腺素3种化合物的优化分离,建立测定中药马齿苋中去甲肾上腺素的简便、快速的分离检测方法。[方法]采用未涂层弹性石英毛细管柱(60 cm×75μm i.d.,有效长度50 cm)作为分离通道,以含8 mmol/Lβ-CD的20 mmol/L硼砂(pH=9.5)为运行缓冲溶液,进样0.5 psi(3.447 kPa)×10 s,分离电压15 kV,检测波长215 nm,柱温25℃。[结果]3种肾上腺素在10 min内得到基线分离,且去氧肾上腺素、肾上腺素和去甲肾上腺素分别在16.74～267.84 mg/L(r=0.999 5),16.18～258.88 mg/L(r=0.999 2),16.181～94.16 mg/L(r=0.999 2)浓度范围内呈良好的线性关系,平均回收率(n=6)分别为99.16%、101.24%和98.96%,RSD均小于2.2%。同时,测得中药马齿苋中去甲肾上腺素的质量分数为0.052%。[结论]该方法简便、快速,可用于肾上腺素类药物的分离和含量测定。
译 名：
Optimization Separation of Three Adrenalines and Quantitative Analysis in Portulaca oleracea L. by Capillary Zone Electrophoresis
作 者：
GAO Su-ya et al(Department of Pharmaceutical Science,Xi'an Medical University,Xian,Shaanxi 710021)
关键词：
Capillary zone electrophoresis(CZE);Adrenalines(ADs);Portulaca oleracea L;Optimization separation;Content determination
摘 要：
[Objective] To develop a capillary zone electrophoresis(CZE) method for optimization separation of three adrenalines and determination of noradrenaline in Portulaca oleracea L.[Method] An uncoated fused-silica capillary column(60 cm×75 μm i.d.,effective length 50 cm) was used as a separation channel.The separation was obtained with 8 mmol/L β-CD in 20 mmol/L borax buffer(pH 9.5) at a constant voltage of 15 kV,the detective wavelength 215 nm and temperature 25 ℃.The sample was injected at 0.5 psi(3.447 kPa)×10 s.[Results] Three compounds were separated baselinely in 10 min.A good linearity between peak area and concentration was found in the range of 16.74-267.84 mg/L(r=0.999 5) for phenylephrine(PE),16.18-258.88 mg/L(r= 0.999 2) for adrenaline(AD),and 16.18-194.16 mg/L(r=0.999 2) for noradrenaline(NE),respectively.Their average recoveries(n=6) were 99.16%,101.24% and 98.96%,respectively,and the relative standard deviation(RSD) was less than 2.2%.This method was used to determine NE in Portulaca oleracea L.The mass fraction was 0.052%.[Conclusion] The method is simple and rapid which could be used to separate and determine ADs.
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