Construction of ubi promoter-based plant expression vector of stilbene synthase gene and transformation of type Ⅱ callus of maize by particle bombardment

GUO Zhi-hong1,WANG Hanning1,TAO Lin2,ZHANG Jin-wen1,BAI Bin1,CHEN Zheng-hua3 (1. Agronomy Faculty of Gansu Agricultural University, Gansu,Lanzhou 730070; 2. College of Enviromental and Municipal Engineering, Gansu, Lanzhou 730070;3. Research Station for Postdoctors of Yasheng Group, Beijing 100101, China)

stilbene synthase gene of peanut;ubi promoter of maize;plant expression vector;maize;genetic transformation

ubi promoter of maize was cloned in our research. pBIUB vector containing this promoter and plant expression vector pBIUG with GFP gene driven by ubi promoter were constructed on the basis of pBI121. pBIUG was introduced into onion epidermis cells by particle bombardment and the results indicated that GFP gene expressed. RT-PCR was employed to clone Stilbene synthase gene of peanut in our experiment. The concensus between the amino acid sequence of the protein the cloned gene encoded and that of Stilbene synthase published was 99.49 % while no mutation occurred at the site of Cys164. Plant expression vector pBIUA, constructed by inserting Res gene into vector pBIUB, was transferred into type II callus of maize and transforments were obtained.