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Position: Home > Articles > Isolation, Purification and Relative Molecular Mass Determination of Corn Bran Polysaccharides FOOD SCIENCE 2010,31 (10) 16-19

玉米皮多糖制备、分离纯化及相对分子质量的测定

作  者:
张艳荣;于君;刘相阳;王大为
单  位:
吉林农业大学食品科学与工程学院
关键词:
玉米皮;多糖;分离纯化;相对分子质量
摘  要:
采用微波提取及95%乙醇沉淀处理制备玉米皮粗多糖,经D315型离子交换树脂脱蛋白,乙醇分级沉淀及SephadexG-100、SephadexG-75层析柱纯化得CSPA-1、CSPA-2和CSPB。紫外-可见光谱在220~400nm处扫描CSPA-1、CSPA-2及CSPB,无蛋白及核酸吸收峰。纯化得到的多糖经葡聚糖凝胶层析法测得相对分子质量分别为186170、103885、146887。
译  名:
Isolation, Purification and Relative Molecular Mass Determination of Corn Bran Polysaccharides
作  者:
ZHANG Yan-rong,YU Jun,LIU Xiang-yang,WANG Da-wei (College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China)
关键词:
corn spermoderm;polysaccharide;purification;relative molecular mass
摘  要:
Crude corn bran polysaccharides (CBP) were obtained through microwave-assisted extraction followed by 95% alcohol precipitation, purified through D315 ion exchange resin column chromatography for the removal of protein and fractionated into 2 fractions, CBPA and CBPB, through fractional precipitation with alcohol. CBPA was further fractionated through Sephadex G-100 column chromatography into 2 subfractions, CBPA-1 and CBPA-2 of which Sephadex G-75 column elution profile exhibited a single absorption peak. Sephadex G-75 column chromatographic analysis indicated that CBPB comprised a single homogeneous composition. Neither protein nor nuclear acid characteristic absorption peaks were shown in the UV-visible spectra of CBPA-1, CBPA-2 and CBPB. The relative molecular mass of CBPA-1, CBPA-2 and CBPB were determined by the dextran gel filtration method to be 186170, 103885 and 146887 , respectively.

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