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Position: Home > Articles > Study on the Physiological Mechanism of the Resistance of Galium aparine L. against Florasulam Journal of Shandong Agricultural University(Natural Science Edition) 2014 (5) 778-781+784

猪殃殃抗双氟磺草胺的生理机制研究

作  者:
马鹏生
单  位:
宁夏大学农学院
关键词:
猪殃殃;双氟磺草胺;ALS活性;保护性酶
摘  要:
本文通过田间试验和室内分析测定,对敏感性猪殃殃(S生物型)和抗性猪殃殃(R生物型)生理指标变化进行了初步研究。试验结果表明:在田间推荐用量上限4.5 g a.i·hm-2双氟磺草胺处理后,R生物型体内的ALS活力表现为先降低后增高趋势,第7 d时达到峰值,是对照的1.4倍。而S生物型体内ALS活力随着处理时间延长也逐渐降低。通过对保护性酶POD、SOD研究结果表明,R生物型喷药后,体内的POD变化呈增高-降低-增高的趋势,第7 d时达到峰值,比对照高16.5 g-1·FW-1,SOD变化趋势则表现为先降低后增高。S生物型在处理后第4 d,体内的POD活性显著下降,7 d时降到最低,而SOD变化则为随着处理时间延长,SOD活性也逐渐降低。综合分析表明,R生物型在受到双氟磺草胺胁迫后,植株能够进行正常生长,靶标酶ALS仍然保持较高的活性,保护性酶POD、SOD持续稳定是对双氟磺草胺产生抗性的主要生理机制。
译  名:
Study on the Physiological Mechanism of the Resistance of Galium aparine L. against Florasulam
作  者:
MA Peng-sheng;School of Agriculture Ningxia University;
关键词:
Galium aparine L.;;Florasulam;;ALS activity;;protective enzymes
摘  要:
This paper preliminarily studied the physiological symptom change of sensitive Galium aparine L.(S biotype) and resistant Galium aparine L.(R biotype) through field test and analysis measure in laboratory. The results showed that: the ALS dynamic performance in R biological increased first then decreased after sulfur difluoride alachlor treatment in the field recommended dosage limit 4.5 g a.i hm-2, and reached a peak at 7 d, which is 1.4 times of references. While in S biological,the ALS dynamic performance gradually reduce with the prolong of processing time. Results through the study on protective enzyme POD and SOD showed that POD changes in R biotype after spraying was increasing, reducing and increasing and reach a peak at 7 d, which is 16.5 g-1 ? FW-1 higher than the reference. SOD changing trend was decreasing first then increasing. S biotype at 4 d after processed. POD activity in the body was significantly decreased after treatment and reached a minimum at 7 d. SOD changes prolonged with the prolonging processing time and SOD activity was also decreased.Comprehensive analysis showed that the plants of R bio-type can normally grow after being stressed by double fluoride sulfur alachlor and neuropathy target esterase ALS remained high activity, the sustained and steady protective enzymes POD,SOD was the main physiological mechanism producing anti-sulfonamide to Florasulam.

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