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当前位置: 首页 > 文章 > 两种落叶松树皮中原花色素的快速提取和光谱分析 林业工程学报 2016,1 (6) 70-74
Position: Home > Articles > Fast extraction and spectroscopy analysis of proanthocyanidins from bark of two larch species Journal of Forestry Engineering 2016,1 (6) 70-74

两种落叶松树皮中原花色素的快速提取和光谱分析

作  者：
何玉婵;李改云;向琴;劳万里
单  位：
中国林业科学研究院木材工业研究所
关键词：
落叶松树皮;原花色素;微波辅助提取;光谱分析
摘  要：
原花色素的提取通常采取用传统外加热方式的溶剂浸渍法,存在提取时间长、提取效率低等缺点,而微波辅助提取技术具有加热效率高、提取时间短和提取率高等优点,成为传统提取技术的一种替代方式。笔者以兴安落叶松和日本落叶松树皮为研究对象,对比研究了微波辅助提取法和常规水浴浸渍法在不同提取时间下对原花色素提取得率的影响,并利用紫外光谱仪和红外光谱仪对树皮提取物的结构进行了表征。结果表明,和常规浸渍法相比,利用微波辅助提取法可显著缩短提取时间,提高原花色素的提取得率,并且不会引起原花色素结构的破坏。兴安落叶松和日本落叶松树皮中原花色素的微波辅助提取适宜工艺条件为:微波功率200 W,提取时间10 min,温度60℃,溶剂为50%乙醇,溶剂和树皮的体积与质量比(mL∶g)为10∶1。在此条件下,兴安落叶松和日本落叶松树皮中原花色素的提取得率分别为239%和278%。两者树皮中的原花色素均以原花青素结构为主,仅含有少量原翠雀素。相同条件下,兴安落叶松树皮比日本落叶松树皮的原花色素提取得率稍低。微波辅助提取法适用于落叶松树皮中原花色素的提取,是一种快速、有效和安全的方法。
译  名：
Fast extraction and spectroscopy analysis of proanthocyanidins from bark of two larch species
作  者：
HE Yuchan;LI Gaiyun;XIANG Qin;LAO Wanli;Research Institute of Wood Industry,Chinese Academy of Forestry;
关键词：
larch bark;;proanthocyanidin;;microwave-assisted extraction;;spectroscopy analysis
摘  要：
Larch bark is a rich source of proanthocyanidin oligomers that may play a key role in the prevention of cardio-vascular and neurodegenerative diseases and cancer. Proanthocyanidins are usually extracted by external heating, whichhas the inherent disadvantages of longer heating time and lower extraction efficiency. Microwave irradiation is a promisingalternative method to conventional heating due to its higher heating efficiency and extraction rate, and reduced extractiontime. In this study, barks ofLarix gmeliniandL. kaempferiwere extracted using microwave heating in comparison to con-ventional water bath heating. The bark extracts were analyzed by UV spectroscopy and infrared spectroscopy. The resultsshowed that microwave-assisted extraction could significantly shorten the extraction time and increase the extraction rateof proanthocyanidins than the conventional water bath heating. Moreover, the structure of proanthocyanidin would not bedestroyed by microwave heating. The optimal conditions for microwave-assisted extraction were obtained as follows:micro-wave power 200 W, extraction time 10 min, temperature 60 ℃, solvent 50% ethanol, solvent to bark ratio 10 ∶1(mL ∶g).The yield of raw proanthocyanidin from bark of bothL.gmeliniandL. kaempferiunder optimal conditions were 23 9%and 27 8%, respectively. The proanthocyanidins from these two larch barks were mainly composed of procyanidin andtrace amount of prodelphinidin. The yield of raw proanthocyanidin from bark ofL. gmeliniwas slightly lower thanL.kaempferiunder the same condition. Microwave-assisted extraction of proanthocyanidin from larch bark is a promisingfast, effective and safe method.

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