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当前位置: 首页 > 文章 > 不同培养条件对甘肃棘豆内生真菌多样性的影响 畜牧兽医学报 2013,44 (10) 1660-1666
Position: Home > Articles > Effect of Different Culture Conditions on Diversity of Endophytic Fungi in Oxytropis kansuensis Chinese Journal of Animal and Veterinary Sciences 2013,44 (10) 1660-1666

不同培养条件对甘肃棘豆内生真菌多样性的影响

作  者：
杨晓雯;赵宝玉;路浩;白玛桑姆;曹丹丹;周启武
单  位：
西北农林科技大学动物医学院
关键词：
疯草;甘肃棘豆;培养条件;内生真菌;多样性
摘  要：
为探讨不同培养条件对中国甘肃棘豆内生真菌多样性的影响,本研究对采集于青海省祁连县甘肃棘豆的茎和叶部,运用表面消毒法将组织块分别置于黑暗和光照条件及PDA和BDA两种培养基上进行内生真菌分离纯化,采用形态学和ITS序列分析技术用于种属鉴定,并通过Biodap软件计算多样性指数。结果显示,629块植物样品中共分离到内生真菌433株,分属9个科15个属28种,不同培养条件优势菌属有所不同,总体而言,Undifilum oxytropis为优势菌属,且光照条件下更加明显;多样性指数计算结果显示,香农-维纳指数(H)为1.99,均匀度指数(E)为0.60,辛普森指数(D)为0.257,不同条件下多样性指数不同,黑暗条件下香农-维纳指数最高,光照条件下辛普森指数最大。结果提示,不同条件下内生真菌的分离率和多样性差异显著,黑暗条件下采用PDA培养基能提高内生真菌的分离率,黑暗条件下采用BDA培养基能增加内生真菌多样性,单就Undifilum oxytropis而言,光照条件下采用BDA培养基能提高其分离率。
译  名：
Effect of Different Culture Conditions on Diversity of Endophytic Fungi in Oxytropis kansuensis
作  者：
YANG Xiao-wen;ZHAO Bao-yu;LU Hao;Bai Ma-sang-mu;CAO Dan-dan;ZHOU Qi-wu;College of Veterinary Medicine,Northwest A&F University;
关键词：
locoweed;;Oxytropis kansuensis;;culture conditions;;endophytic fungi;;diversity
摘  要：
To ascertain the effect of different culture conditions on diversity of endophytic fungi fromOxytropis kansuensis in China,the study collected Oxytropis kansuensis samples in Qilian county of Qinghai province,using the method of surface disinfection and different medium,to isolate and purify the endophytic fungi in stems and leafs,then the morphology techniques and ITS sequence analysis were used to identify their species,and calculated diversity index by Biodap.The results showed that 433 fungal colonies were isolated from 629 samples,which were combined to 28 kinds of different endophytic fungus.Undifilum oxytropis was the dominant strain,and advantage was more obvious in the light condition.The Shannon-Weiner index was1.99,evenness index was 0.60,simpson'diversity index(D)was 0.257.The diversity was different under different culture conditions.The Shannon-Weiner index was highest in the dark condition,and the simpson'diversity index was biggest in the light condition.The results showed that the diversity and isolation rate of endophytic fungi from Qilian county of Qinghai province was different under different culture conditions.Dark condition and PDA medium could improve the isolation rate of endophytic fungi,dark condition and BDA medium can increase the diversity,as the Undifilum oxytropis,the light condition and BDA medium can increase the separation rate.

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