Position: Home > Articles > Study of Vegetative Storage Protein in Euonymus fortunei var.Radicans
Northern Horticulture
2009
(5)
74-75
爬行卫矛贮藏蛋白的初步研究
作 者：
孟春晓;高政权
单 位：
山东理工大学生命科学学院
关键词：
爬行卫矛;贮藏蛋白;显微观察;SDS-PAGE
摘 要：
以不同季节的爬行卫矛枝条为研究对象,利用光学显微技术和SDS-PAGE技术来研究其休眠期和生长期枝条中贮藏蛋白的分布情况。经汞—溴酚蓝染色后的石蜡切片显微观察表明:休眠期枝条细胞的液泡中有大量蓝色小球状蛋白颗粒,有的甚至充满整个液泡,而在生长期枝条细胞中则未发现这种蛋白颗粒的存在。SDS-PAGE结果表明:与生长期枝条样品相比,有一种分子量约为45 kD蛋白质在休眠期枝条大量特异表达,这可能就是贮藏蛋白。
译 名：
Study of Vegetative Storage Protein in Euonymus fortunei var.Radicans
作 者：
MENG Chun-xiao,GAO Zheng-quan (School of Life Sciences,Shandong University of Technology,Zibo,Shandong 255049,China)
关键词：
Euonymus fortunei var Radicans;Vegetative storage proteins;Microscopy;SDS-PAGE
摘 要：
Vegetative storage proteins(VSPs)in the Euonymus fortunei var.Radicans were identified by using optical microscopy and SDSPAGE.Under optical microscope,deep blue masses in a granular appearance were observed in the secondary xylem parenchyma cells of the dormancy season barks after stained with mercury-bromophenol blue.However,there was no granular protein in the development season barks.Compared with SDS-PAGE results of the development season barks,there was a 45 kD major protein expressed in the dormancy season barks,which was probably the vegetative storage protein of Euonymus fortunei var.Radicans.
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