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当前位置: 首页 > 文章 > 楠木种属及其近缘属叶绿体matK基因序列的鉴定 贵州农业科学 2014,42 (2) 27-31
Position: Home > Articles > Identification of Chloroplast matK Sequence of Phoebe and Its Related Genera Guizhou Agricultural Sciences 2014,42 (2) 27-31

楠木种属及其近缘属叶绿体matK基因序列的鉴定

作  者：
陈云霞;南程慧;薛晓明
单  位：
南京森林警察学院
关键词：
楠木;近缘属;鉴定;matK
摘  要：
为给楠木及其近缘属植物的DNA分子鉴定提供依据,对30种楠木及其近缘属植物进行基因组DNA提取,叶绿体matK序列扩增与测定,并进行序列比对与人工校正,计算属间及种间的遗传距离,比较序列间的差异,构建系统发育树。结果表明:matK各基因序列的组成中G+C含量平均为36.6%。30个楠木及近缘属植物共发现72个碱基变异位点,碱基变异率为9.38%。其matK基因序列种间遗传距离为0~0.098,平均遗传距离为0.010。聚类分析,新樟属为1个分支,楠属和润楠属聚为1个分支,其余樟属、木姜子属、新木姜子属、山胡椒属、檫木属、月桂属与黄肉楠属聚为1个分支。故matK序列基本可以对楠木及其近缘属植物进行属间鉴定,但在种间水平上并不可行。
译  名：
Identification of Chloroplast matK Sequence of Phoebe and Its Related Genera
作  者：
CHEN Yunxia;NAN Chenghui;XUE Xiaoming;Nanjing Forest Police College;
关键词：
Phoebe;;related genera;;identification;;matK
摘  要：
To provide references for DNA identification of Phoebe and its related genera,genomic DNA was extracted from 30accessions,the chloroplast matKsequences were analyzed,the inter-specific genetic distances were calculated,and the phylogenetic trees of all the accessions tested were constructed based on the obtained distance data.The results indicated that the average G+C content in various matKsequences was 36.6%.72base variation points were found,accounting for 9.38%.The interspecific genetic distance ranged from 0to 0.098,with an average of 0.010.The cluster analysis showed that the genera tested were clustered into three branches,one was Neocinnamomum delavayi,one was Phoebe and Machilus,and others belonged to another one,including Cinnamomum,Litsea,Neolitsea,Lindera,Sassafras,Laurus and Actinodaphne.matKcould be used to identify Phoebe and its related genera,but couldn't be used at the species level.

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