Position: Home > Articles > Determination of plasma concentration of PNS-β by SPE-HPLC and its pharmacokinetic in rats
Journal of Gansu Agricultural University
2014
(6)
29-34
SPE-HPLC法测定三七皂苷β-环糊精包合物血药浓度及在大鼠体内的药动学研究
作 者：
郑义;陈晓兰;丁宁;黄琳;陆辉
单 位：
江苏农牧科技职业学院
关键词：
β-环糊精包合物;人参皂苷Rg1;药动学;大鼠
摘 要：
采用固相萃取-高效液相色谱(SPE-HPLC法)测定PNS-β中人参皂苷Rgl的浓度,以3P97药代动力学程序计算药代动力学参数,比较PNS-β和PNS在静脉注射和灌胃给药条件下在大鼠体内的药动学特征.结果表明:PNS-β经大鼠灌胃给药后,其吸收半衰期(T1/2ka)为(0.48±0.12)h,最大血药浓度(Cmax)为(15.91±1.17)μg/mL,消除半衰期(T1/2ke)为(7.38±0.52)h,生物利用度(F%)为50.81%,而PNS经大鼠灌胃给药后,其吸收半衰期(T1/2ka)为(0.55±0.06)h,最大血药浓度(Cmax)为(5.32±0.16)μg/mL,消除半衰期(T1/2ke)为(4.89±0.39)h,生物利用度(F%)为18.43%.PNS-β环糊精包合物口服给药,吸收迅速,能达到较高的血药浓度,消除缓慢,药物在体内维持时间长,不仅达到药物缓释的效果,还提高了PNS口服给药的生物利用度.
译 名：
Determination of plasma concentration of PNS-β by SPE-HPLC and its pharmacokinetic in rats
作 者：
ZHENG Yi;CHEN Xiao-lan;DING Ning;HUANG Lin;LU Hui;Vocational College of Jiangsu Agri-animal Husbandry;
关键词：
PNS-β;;ginsenoside Rg1;;pharmacokinetic;;rats
摘 要：
The concentration of ginsenoside Rg1 in PNS-βwas measured by the SPE-HPLC method;the pharmacokinetic parameters were obtained by using 3P97 pharmacokinetics program;and the pharmacokinetic features of PNS-βand PNS in rats by intravenous injection and intragastric infusion with two different dose conditions were compared.The results indicated that when the rats were administered with PNS orally,T1/2ka,Cmax,T1/2ke and F% were(0.48±0.12)h,(15.91±1.17)μg/mL,(7.38±0.52)h and50.81%respectively;when the rats were administered with PNS-βorally,T1/2ka,Cmax,T1/2ke and F%were(0.55±0.06)h,(5.32±0.16)μg/mL,(4.89±0.39)h and 18.43% respectively.It is suggested that PNS-βadministered orally could be absorbed quickly,reach a high plasma concentration,eliminate slowly and maintaine a long time in body,the chemical has sustained-release effects and could improve bioavailability of oral administration.
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