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当前位置: 首页 > 文章 > 免疫亲和柱净化法对奶酪中黄曲霉毒素的检测 南京林业大学学报(自然科学版) 2006,30 (1) 76-78
Position: Home > Articles > Immunoaffinity Column Clean-up of Five Aflatoxins from Cheese and Analysis by HPLC Journal of Nanjing Forestry University(Natural Sciences Edition) 2006,30 (1) 76-78

免疫亲和柱净化法对奶酪中黄曲霉毒素的检测

作  者：
曾义;叶建仁
单  位：
淮安出入境检验检疫局;南京林业大学森林资源与环境学院
关键词：
HPLC;梯度洗脱;奶酪;黄曲霉毒素;免疫亲和柱
摘  要：
采用免疫亲和柱净化-反相HPLC法,对加标的奶酪样品中的黄曲霉毒素B1、B2、G2、G1和M1进行了检测,并运用梯度洗脱技术优化了色谱条件。结果表明:G1、B1和M1的线性范围为2~20μg/L,而B2和G2线性范围为0.6~6.0μg/L。B1、B2、G1、G2和M1的检出限分别为0.2、0.3、0.06、0.1和0.4μg/L;各组分在3个不同加标水平上的平均回收率都大于76%;相对标准偏差在1.7%~4.5%。
译  名：
Immunoaffinity Column Clean-up of Five Aflatoxins from Cheese and Analysis by HPLC
作  者：
ZENG Yi~1,YE Jian-ren~2(1.Huaian Entry-exit Commodity Inspection and Quarantine Bureau,Huaian 223001,China;2.College of ForestResources and Environment Nanjing Forestry University,Nanjing 210037,China)
关键词：
HPLC;Gradient elution;Cheese;Aflatoxins;Immunoaffinity column
摘  要：
A method based on immunoaffinity column clean-up and reversed-phase high-(performance) liquid chromatography(RP-HPLC) with fluorescence detection has been applied to determinate aflatoxins B1,B2,G1,G2 and M1 from spiked cheese.Chromatographic conditions were bettered by gradient elution technique.Calibration curves of aflatoxins G1,B1 and M1 were linear up to 2~20μg/L,and the calibration curves of aflatoxins G2 and B2 were linear up to 0.6~6.0μg/L.Limits of determination of B1,B2,G1,G2 and M1 were 0.2,0.3, 0.06,0.1 and 0.4μg/L,respectively.Average recoveries of the aflatoxins Bl,B2,G1,G2 and M1 from cheese spiked with three different concentration levels were greater than 76%,and the RSDs ranged from 1.7% to 4.5%.

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