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当前位置: 首页 > 文章 > MdMYB73的分子克隆及其在苹果愈伤组织和拟南芥幼苗中的盐抗性功能鉴定 园艺学报 2016,43 (11) 2073-2080
Position: Home > Articles > Molecular Cloning and Functional Characterization of MdMYB73 Reveals Its Involvement in Salt Tolerance in Apple Callus and Arabidopsis Acta Horticulturae Sinica 2016,43 (11) 2073-2080

MdMYB73的分子克隆及其在苹果愈伤组织和拟南芥幼苗中的盐抗性功能鉴定

作  者：
张全艳;刘晓;于建强;胡大刚;郝玉金
单  位：
山东农业大学园艺科学与工程学院作物生物学国家重点实验室农业部黄淮地区园艺作物生物学与种质创制重点实验室
关键词：
苹果;MYB;MdMYB73;SOS;盐胁迫;基因表达
摘  要：
从‘嘎拉’苹果中克隆了一个MYB转录因子基因(序列号:MDP0000894463)。该基因包含长为729 bp完整的开放阅读框,编码243个氨基酸,预测其蛋白质分子量为26.34 kD,等电点为9.29。系统进化树分析表明,这一MYB转录因子与拟南芥AtMYB73同源序列相似性最高,因此将其命名为MdMYB73。功能域分析表明,MdMYB73蛋白含有保守的R2R3-typeMYB绑定域。荧光定量PCR分析表明,MdMYB73在苹果的各个组织均有表达,在叶片和花中表达相对较高;MdMYB73的表达明显受盐胁迫的诱导。将异位表达MdMYB73的拟南芥幼苗进行抗盐鉴定,结果表明MdMYB73负调控拟南芥盐胁迫抗性;同时,AtSOS1,AtSOS3和AtNHX1抗盐相关基因的表达水平显著降低,表明MdMYB73可能负调控SOS反应,影响拟南芥抵抗高盐胁迫过程。将MdMYB73基因遗传转化苹果愈伤组织,抗盐表型分析表明,MdMYB73过量表达也明显降低了转基因愈伤组织对盐胁迫的抗性。
译  名：
Molecular Cloning and Functional Characterization of MdMYB73 Reveals Its Involvement in Salt Tolerance in Apple Callus and Arabidopsis
作  者：
ZHANG Quan-yan;LIU Xiao;YU Jian-qiang;HU Da-gang;HAO Yu-jin;State Key Laboratory of Crop Biology,MOA Key Laboratory of Horticultural Crop Biology(Huanghuai Region)and Germplasm Innovation,College of Horticulture Science and Engineering,Shandong Agricultural University;
关键词：
apple;;MYB;;MdMYB73;;SOS;;salt stress;;gene expression
摘  要：
A MYB transcription factor(TF)(Gen Bank accession number:MDP0000894463)was cloned from Malus × domestica‘Royal Gala'. Sequence analysis showed that the length of this gene was 729 bp,which encoded 243 amino acids. It was predicted that the molecular mass of this protein was 26.34 kD,and pI was 9.29. Phylogenetic tree indicated that our cloned apple MYB TF exhibited the highest sequence similarity to Arabidopsis AtMYB73,so named MdMYB73. Analysis of functional domain showed that the MdMYB73 protein included the conserved R2R3-type MYB domain. Quantitative real-time PCR(qRT-PCR)analysis demonstrated that the MdMYB73 gene was extensively expressed in different tissues and organs of apple. Specially,the expression level of MdMYB73 was relatively higher inapple leaves and flowers. Meanwhile,the expression of MdMYB73 was obviously induced by salt stress. In addition,ectopic expression of MdMYB73 decreased salt stress resistance in Arabidopsis,indicating that MdMYB73 negatively regulated the salt tolerance. qRT-PCR analysis showed that the expression levels of salt stress-related genes AtSOS1, AtSOS3 and AtNHX1 were significantly decreased in transgenic Arabidopsis plants,suggesting that MdMYB73 negatively regulated SOS pathway. Finally,salt-tolerance assay indicated that overexpression of MdMYB73 remarkably decreased the tolerance of transgenic apple callus to high salinity,further supporting that MdMYB73 negatively control salt tolerance in apple.

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