Development of an Indirect-ELISA for HRV and HRV Infection Rates Investigation of Children Diarrhea in Daqing city

Wen Cheng1,2,Wang Wei3,Yu Liyun1,Hou Xilin4,Shan Chunqiao1,Tan Jun4,Sun Jingxiu1(1.College of Life Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319;2.Daqing Medical College; 3.General Hospital of Daqing Oil Field;4.College of Animal Science and Technology,Heilongjiang Bayi Agricultural University)

human rotavirus;isolation and identification;indirect-ELISA;infection rates

In order to monitor human rotavirus(HRV) infection with children in daqing city recent,a local epidemic strains of HRV that Had been separated and identified was used,then differential centrifugation was performed to concentrate and purify the HRV.The purified HRV serving as coating antigen,according to the indirect ELISA procedures set test in order to determin and optimize the optimal conditions of this method.Thus an indirect ELISA method was established to detect HRV.Preliminary epidemiological study was performed on infection with rotavirus in 1 452 children with diarrhea in Daqing between July 2011 and March 2012,using the indirect ELISA diagnostic method established in this trial,and the statistical analysis was performed on HRV infection rates test results according to season,children's age,region,gender etc.The results showed that the indirect ELISA method established in this trial had high sensitivity,high specificity and good repeatability,and the results is sensitive and accurate.1 452 cases of children of the diarrhea was detect using the indirect ELISA diagnostic method established in this trial.The results showed that the infection rate of HRV was 39.9% at average,and indicating that children were susceptible to HRV in the autumn and the winter,and children below 3 years were susceptible to HRV.In addition,HRV infection had a certain correlation with Health conditions,economic status and immunity etc.