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当前位置: 首页 > 文章 > 鸭儿芹异黄酮2'-羟化酶基因的克隆及其表达的温度响应 江苏农业学报 2016,32 (3) 640-646
Position: Home > Articles > Cloning of isoflavone 2'-hydroxylase gene from Cryptotaenia japonica Hassk and its expression in response to temperature Jiangsu Journal of Agricultural Sciences 2016,32 (3) 640-646

鸭儿芹异黄酮2'-羟化酶基因的克隆及其表达的温度响应

作  者：
刘洁霞;谭国飞;吴雪君;许瀛之;陈佳凯;熊爱生
单  位：
南京农业大学园艺学院作物遗传与种质创新国家重点实验室
关键词：
鸭儿芹;CjI2'H基因;克隆;实时荧光定量PCR;温度胁迫;基因表达
摘  要：
以贵州野生鸭儿芹为材料,研究了与植物抗逆境胁迫有关的细胞色素P450(CYP450)家族成员异黄酮2'-羟化酶(I2'H)基因对温度胁迫的应答和响应。利用PCR扩增技术克隆得到鸭儿芹异黄酮2'-羟化酶基因Cj I2'H,该基因的开放阅读框长为1 5 2 7 bp,可编码5 0 8个氨基酸,通过DNAMAN软件预测其蛋白质分子质量为1.270 9×10~5,等电点(p I)为4.80。进化树分析结果表明Cj I2'H编码的氨基酸序列在不同植物间相对保守。实时定量PCR结果显示鸭儿芹在30℃处理下,叶柄中Cj I2'H基因的相对表达量随处理时间的增加而上升,且在24 h时达到峰值;10℃处理下,Cj I2'H基因的相对表达量明显低于高温处理。说明Cj I2'H基因表达与温度胁迫存在关联性,高温胁迫后该基因的表达响应大于低温胁迫。
译  名：
Cloning of isoflavone 2'-hydroxylase gene from Cryptotaenia japonica Hassk and its expression in response to temperature
作  者：
LIU Jie-xia;TAN Guo-fei;WU Xue-jun;XU Ying-zhi;CHEN Jia-kai;XIONG Ai-sheng;State Key Laboratory of Crop Genetics and Germplasm Enhancement,College of Horticulture,Nanjing Agricultural University;
关键词：
Cryptotaenia japonica Hassk;;CjI2'H gene;;cloning;;quantitative real-time PCR;;temperature stress;;gene expression
摘  要：
The Cj I2' H gene encoding isoflavone 2'-hydroxylase( I2' H),a member of the cytochrome P450 superfamily related to abiotic stress in plants,was cloned by PCR amplification from wild Cryptotaenia japonica Hassk of Guizhou province. It' s ORF( open reading frame) length is 1 527 bp,encoding 508 amino acids. The enzyme molecular weight predicted by DNAMAN software was 1. 270 9 × 10~5,and the p I was 4.80. The phylogenetic analysis showed that the Cj I2' H-encoded amino acid sequences of I2' H were conservative in different plant species. The expression level of Cj I2' H gene in the petiole of C. japonica Hassk at 30 ℃ detected by quantitative real-time PCR were up-regulated and peaked at 24 h. At 10 ℃,the expression level of Cj I2' H was lower than that at high temperature. The expression of Cj I2' H gene is related to temperature in C. japonica Hassk,greater under high temperature stress.

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