作 者：
华梅;王垚;肖支叶;原晓龙;虞泓;王娟;杨宇明;王毅
单 位：
云南省林业科学院云南省森林植物培育与开发利用重点实验室/国家林业局云南珍稀濒特森林植物保护和繁育实验室;云南大学中草药生物资源研究所云百草实验室;西南林业大学材料工程学院
关键词：
贵州绿僵菌;单菌多产物;抗菌活性;液体培养;稳定性
摘 要：
为了开发虫生真菌潜在的抗菌药用价值,寻找结构新颖的活性化合物,本研究采用单菌多产物策略(OSMAC),研究不同碳源、氮源、接种量、接种时间对贵州绿僵菌菌丝体提取物抑菌活性的影响。结果表明:贵州绿僵菌提取物能对13种常见致病细菌中的蜡样芽孢杆菌、缓慢芽孢杆菌和溶血性葡萄球菌产生抑制作用,提取物最低抑菌浓度分别为6.25、1.56、6.25 mg/m L;不同碳源和氮源对贵州绿僵菌液体培养提取物的抑菌活性影响显著,其中淀粉和马铃薯培养液提取物的抑菌效果最好;不同接种量对贵州绿僵菌提取物抑菌效果的影响不大;随着培养时间的增加,抑菌活性依次增强。
译 名：
Antibacterial Activities of Liquid Culture Extracts of Metarhizium guizhouense
作 者：
Hua Mei;Wang Yao;Xiao Zhiye;Yuan Xiaolong;Yu Hong;Wang Juan;Yang Yuming;Wang Yi;Yunnan Provincial Key Laboratory of Cultivation and Exploitation of Forest Plants/Key Laboratory for Conservation of Rare,Endangered & Endemic Forest Plants,State Forestry Administration,Yunnan Academy of Forestry;Yunnan Herbal Laboratory,Institute of Herb Biotic Resources,Yunnan University;College of Materials Engineering,Southwest Forestry University;
关键词：
Metarhizium guizhouense;;OSMAC;;antibacterial activities;;liquid culture;;stability
摘 要：
In order to explore the potential antibacterial medicinal value of insect fungi and find new compounds with novel structures and bioactivity.In this study,the single-cell multi-product strategy(OSMAC) activates were used in liquid culture of Metarhizium guizhouense.The effects of different carbon sources,nitrogen sources,inoculation amount and inoculation time on antibacterial activity of liquid culture extracts of Metarhizium guizhouense were detected.The results showed that the extracts of Metarhizium guizhouense could inhibit the growth of Bacillus cereus,Bacillus lentus and Straphylococcus haemolyticus of 13 common pathogenic bacteria.The minimum inhibitory concentrations of the extracts to 3 pathogenic bacteria were 6.25 mg/mL,1.56 mg/mL,6.25 mg/mL,respectively.The effects of different carbon and nitrogen sources on the antibacterial activity of liquid culture extracts of Metarhizium guizhouense was significant,and the extracts of Metarhizium guizhouense grown in starch and potato culture medium showed strongest antibacterial activity.Different inoculation amount had little effects on the antibacterial of extracts of Metarhizium guizhouense.The effects of different culture time on the antibacterial effect of mycelium extract was significant.The antibacterial activity of extract of Metarhizium guizhouense increased with the increase of culture time.
