单 位：
中国海洋大学食品科学与工程学院;青岛工学院食品工程学院
关键词：
冰岛刺参;岩藻糖基化硫酸软骨素;血糖;胰岛素抵抗;脂肪细胞因子
摘 要：
以高脂高糖饲料(high-fat high-sucrose,HFSD)饲喂法建立胰岛素抵抗小鼠模型。研究了冰岛刺参岩藻糖基化硫酸软骨素(fucosylated chondroitin sulfate from the sea cucumber Cucumaria frondosa,Cf-CHS)对胰岛素抵抗小鼠的降血糖及改善胰岛素抵抗作用。雄性C57BL/6J小鼠随机分为正常对照(标准饲料)、模型对照(HFSD)、阳性对照(HFSD+rosiglitazone(RSG),1mg·(kg·d)-1)、Cf-CHS组(HFSD+Cf-CHS,80mg·(kg·d)-1)及Cf-CHS+RSG组(HFSD+Cf-CHS+RSG,80+1mg·(kg·d)-1)。各组小鼠自由摄食摄水19周。实验结束后,称重小鼠白色脂肪质量,检测空腹血糖、血清胰岛素及血清脂联素、抵抗素、瘦素、肿瘤坏死因子-α(TNF-α)水平。实验结果表明:Cf-CHS可显著降低胰岛素抵抗小鼠的脂肪积累(p<0.01),降低血糖(p<0.01)和胰岛素(p<0.05)水平,改善胰岛素抵抗(p<0.05),提高血清脂联素含量(p<0.05),降低抵抗素(p<0.01)、瘦素(p<0.01)和TNF-α(p<0.05)含量。Cf-CHS与RSG复配使用,效果更显著(p<0.05,p<0.01)。Cf-CHS能显著改善胰岛素抵抗小鼠的高血糖症状及胰岛素抵抗程度,其作用机制可能与改善肥胖引起的脂肪细胞因子的分泌紊乱有关。
译 名：
Study of fucosylated chondroitin sulfate from Cucumaria frondosa on hyperglycemic effects and insulin resistance improvement
作 者：
TIAN Ying-ying;HU Shi-wei;XUE Chang-hu;LI Zhao-jie;College of Food Engineering,Qingdao University of Technology;College of Food Science and Engineering,Ocean University of China;
关键词：
Cucumaria frondosa;;fucosylated chondroitin sulfate from sea cucumber;;blood glucose;;insulin resistance;;adipocytokines
摘 要：
The model mice was established by feeding a diet of high-fat high-sucrose diet( HFSD).The aim of the present study was to unravel the effects of fucosylated chondroitin sulfate from Cucumaria frondosa( Cf-CHS) on hyperglycemic effects and insulin resistance improvement in insulin resistant mice. Male C57BL /6 mice were randomly assigned into the normal control( standard diet),model control( HFSD),positive control( HFSD +rosiglitazone( RSG),1mg·( kg·d)- 1),Cf-CHS( HFSD + + Cf-CHS,80mg·( kg·d)- 1),Cf-CHS + RSG( HFSD + Cf-CHS + RSG,80 + 1mg·( kg·d)- 1) groups. All animals fed with diet and water freely for 19 weeks. After experiments,the white fat weight,fasting serum glucose,serum insulin,serum adiponectin,resistin,leptin,and tumor necrosis factor- α( TNF- α) levels were determined. Results showed that Cf- CHS markedly decreased fat deposition( p < 0.01),blood sugar( p < 0.01) and insulin( p < 0.05) levels,ameliorated insulin resistance( p < 0.05),increased serum adiponectin concentration( p < 0.05),and lowered serum resistin( p < 0.01),leptin( p < 0.01),TNF-α( p < 0.05) levels.The effect was more significant when Cf- CHS compounded with RSG( p < 0.05,p < 0.01).Cf-CHS significantly decreased blood glucose level and ameliorated insulin resistance in insulin resistant mice,and the mechanisms may involved the regulation of adipocytokines parasecretion caused by adiposity.
