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当前位置: 首页 > 文章 > 食源性金黄色葡萄糖球菌耐氨基糖苷类药物基因的多重PCR快速检测技术 沈阳农业大学学报 2015,46 (1) 31-36
Position: Home > Articles > Establishment of Detection of Oxacillin and Aminoglycoside Resistance Genes in Foodborne Staphylococcus aureus by Multiplex PCR Journal of Shenyang Agricultural University 2015,46 (1) 31-36

食源性金黄色葡萄糖球菌耐氨基糖苷类药物基因的多重PCR快速检测技术

作  者：
战晓微;郑秋月;傅俊范;徐扬;那晗
单  位：
辽宁出入境检验检验局;沈阳农业大学植物保护学院
关键词：
金黄色葡萄球菌;MRSA;氨基糖苷类抗生素;耐药基因;多重PCR
摘  要：
为研究食源性金黄色葡萄球菌中MRSA及对氨基糖苷类庆大霉素、卡那霉素抗生素的耐药性情况,并建立快速检测鉴定金黄色葡萄球菌耐药性多重PCR方法。对金黄色葡萄球菌进行增菌培养,并提取DNA作为模板,以nuc基因作为菌属鉴定基因,mecA基因作为MRSA检测基因,aac(6')-aph(2'')及aph(3')-Ⅲa基因作为耐氨基糖苷类庆大霉素、卡那霉素和妥布霉素的检测目的基因,建立四重PCR检测方法并进行特异性及灵敏度评价;利用所建立的方法检测19株金黄色葡萄球菌的分离株。结果表明:对19株金黄色葡萄球菌分离株的PCR检测结果与纸片扩散法的检测结果一致。所建立的多重PCR方法灵敏度高、特异性好,可以快速检测出MRSA,并对氨基糖苷类庆大霉素、卡那霉素、妥布霉素耐药菌株检测具有较高的准确率。
译  名：
Establishment of Detection of Oxacillin and Aminoglycoside Resistance Genes in Foodborne Staphylococcus aureus by Multiplex PCR
作  者：
ZHAN Xiao-wei;ZHENG Qiu-yue;FU Jun-fan;XU Yang;NA Han;College of Plant Protection,Shenyang Agricultural University;Liaoning Entry-exit Inspection and Quarantine Bureau;
关键词：
Staphylococcus aureus;;MRSA;;aminoglycoside;;resistant gene;;multiplex PCR
摘  要：
The aim of this experiment was to investigate the prevalence of MRSA and resistance to aminoglycoside gentamicin kanamycin and tobramycin in foodborne Staphylococcus aureus, and establish a rapid detection and identification antibiotic resistance of Staphylococcus aureus method by Multiple-PCR. Genetic DNA was extracted as template for PCR from Staphylococcus aureus collected and cultured in enrichment medium. According to nuc for Genus, mecA for MRSA, aac(6')-aph(2'') and aph(3')-Ⅲa for gentamicin, kanamycin and tobramycin, quadruple RCR detection method was developed, sensitivity and specificity were evaluated. 19 strains of Staphylococcus aureus from food and patients with food poisoning were detected by quadruple RCR method. Quadruple RCR detection method was established. The result of quadruple RCR detection for the 19 strains of Staphylococcus aureus were consistent with disk diffusion testing. This multi-PCR method is specific, sensitive, and provides a rapid detection for MRSA, gentamicin, kanamycin and tobramycin resistant strains.

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