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Position: Home > Articles > Electronic Clone and Bioinformatics Analysis of F-box Protein Gene from Wheat(Triticum aestivum L.) Journal of Henan Agricultural Sciences 2013,42 (2) 15-19,25

小麦F-box蛋白基因的电子克隆和生物信息学分析

作  者:
王俊生;李俐俐;杨欢;谭光轩;殷贵鸿
单  位:
周口师范学院生命科学系
关键词:
小麦;F-box蛋白基因;电子克隆;生物信息学
摘  要:
为探讨F-box蛋白在小麦发育过程中的调节作用,利用电子克隆技术获得了一个小麦F-box蛋白基因,采用生物信息学方法,对其编码的蛋白质从序列特征、理化性质、跨膜结构域、亚细胞定位、高级结构及功能域等方面进行了预测和分析.结果表明,小麦F-box蛋白由362个氨基酸组成,在N端和C端分别存在一个F-box保守结构域和一个DUF295保守结构域(功能未知),该蛋白质二级结构中以α-螺旋(23.48%)、扩展束(25.41%)和无规则卷曲(47.24%)为主,在94-115位点处可能存在一个由内向外的跨膜区,N端不存在信号肽序列,亚细胞定位于细胞质中;该F-box蛋白与二穗短柄草、玉米和水稻的F-box蛋白相似性较高,进化距离较近.
译  名:
Electronic Clone and Bioinformatics Analysis of F-box Protein Gene from Wheat(Triticum aestivum L.)
单  位:
Department of Life Sciences,Zhoukou Normal University,Zhoukou 466001,China
关键词:
Triticum aestivum L.%F-box protein gene%electronic clone%bioinformatics
摘  要:
In the paper,an F-box protein gene from wheat was obtained by electronic clone technique,and some characters of the F-box protein were analyzed and predicted by bioinformatics method,including the composition of amino acid sequence,hydrophobicity,secondary structure and function of protein etc.The results showed that the F-box protein was composed of 362 amino acids,and contained an F-box domain in N end and a DUF295 conserved domain in C end of protein sequence.The secondary structure of the F-box protein mainly included α-helix(23.48%),extended strand(25.41%) and random coil(47.24%).The F-box protein had higher sequece indentities and closer evolution distance with that from other Gramineae plants including Brachypodium distachyon,Oryza sativa and Zea may.

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