当前位置: 首页 > 文章 > 不同预处理对花生根尖细胞有丝分裂制片的影响 花生学报 2008,37 (2) 30-33
Position: Home > Articles > Effects of Pretreatment on Mitosis of Root Tip Cells in Peanut Journal of Peanut Science 2008,37 (2) 30-33

不同预处理对花生根尖细胞有丝分裂制片的影响

作  者:
任艳;王辉;石延茂;李双铃;袁美
单  位:
山东省花生研究所国家花生工程技术研究中心
关键词:
花生;预处理;有丝分裂;秋水仙素;8-羟基喹啉;对二氯苯饱和水溶液
摘  要:
以花生根尖为材料,分别用不同质量浓度的秋水仙素(0,0.1,0.5,1.0,1.5,2.0g/L)、8-羟基喹啉(0,2,4,6,8,10mmol/L)和对二氯苯饱和水溶液预处理1~3h,分析了不同预处理剂对花生根尖细胞有丝分裂活性的影响,并比较了不同预处理剂对中期染色体形态的影响。结果表明,各预处理剂在不同浓度下对花生根尖细胞中期分裂指数和中期染色体形态的影响均不相同。用对二氯苯饱和水溶液处理时间2.5 h效果最好,获得了分散均匀的花生根尖细胞染色体的中期分裂相。
译  名:
Effects of Pretreatment on Mitosis of Root Tip Cells in Peanut
作  者:
REN Yan,WANG Hui,SHI Yan-mao,LI Shuan-ling,YUAN Mei(Shandong Peanut Research Institute,National Center for Peanut Engineeringand Technology,Qingdao 266100,China)
关键词:
peanut;pretreatment;mitosis;colchicine;8-Hydroxyquinoline;saturated p-Dichlorobenzene solution
摘  要:
The effects of different pretreatments on mitosis of peanut's root tip cells were investigated under conditions of different concentration of colchicine(0,0.1,0.5,1.0,1.5,2.0g/L),8-Hydroxyquinoline(8-HQ)(0,2,4,6,8,10mmol/L) and saturated p-Dichlorobenzene solution for 1~3 h.The results showed that there existed different influences on metaphase mitotic index and morphlogy of peanut chromosome in pretreatments and corresponding treatment periods.The results indicated that the pretreatment of p-Dichlorobenzene for 2.5 hours are optimum for obtaining chromosomes specimen.

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