当前位置: 首页 > 文章 > 石榴果实成熟期不同品种果皮蛋白质表达的双向电泳分析 果树学报 2015,32 (6) 1062-1069
Position: Home > Articles > Comparative proteomics analysis of pomegranate peel in fruit maturation period Journal of Fruit Science 2015,32 (6) 1062-1069

石榴果实成熟期不同品种果皮蛋白质表达的双向电泳分析

作  者:
曹尚银;牛娟;曹达;李好先;薛辉;陈利娜;张富红;赵弟广
单  位:
中国农业科学院郑州果树研究所;河南师范大学
关键词:
石榴;果皮;成熟期;蛋白质组学;2-DE
摘  要:
【目的】石榴果实色泽是影响果品商品价值的重要因素之一,但石榴果皮颜色形成的分子机制还不清楚。应用差异蛋白组学技术,从蛋白水平上探究石榴果实发育成熟期果皮的代谢进程。【方法】运用双向电泳和MALDI-TOFTOF MS质谱鉴定技术研究‘三白’石榴和‘中农红’石榴果实成熟时果皮蛋白质组学差异。【结果】分别对‘三白’、‘中农红’石榴进行2-DE和质谱鉴定,在‘三白’和‘中农红’石榴果皮的电泳图谱中发现每一张重复胶上分别可以检测到892个蛋白点和890个蛋白点,与‘中农红’相比,在‘三白’果皮图谱中共检测到36个表达量相差2倍以上的蛋白质点,相对于‘中农红’,‘三白’有13个蛋白质点上调表达,19个蛋白质点下调表达。通过质谱鉴定和数据库检索注释了4个蛋白质点。分别为半胱氨酸合酶1(sport 1402)、细胞色素b6-f复合体(sport 0013)、叶绿素a/b结合蛋白(sport 5108)及果糖激酶2-like(sport 0503)。【结论】细胞色素b6-f复合体(sport 0013)、叶绿素a/b结合蛋白(sport 5108)和果糖激酶2-like(sport 0503)可能与果皮颜色变化相关;半胱氨酸合酶1(sport 1402)可能对提高石榴的抗氧化胁迫能力起到了重要作用。为研究石榴果实发育蛋白质组学及改善石榴果实品质提供一定的理论依据。
译  名:
Comparative proteomics analysis of pomegranate peel in fruit maturation period
作  者:
CAO Shangyin;NIU Juan;CAO Da;LI Haoxian;XUE Hui;CHEN Lina;ZHANG Fuhong;ZHAO Diguang;Zhengzhou Fruit Research Institute,Chinese Academy of Agriculture Sciences;Henan Normal University;
关键词:
Pomegranate;;Fruit peel;;Maturation period;;Proteomics;;2-DE
摘  要:
【Objective】Fruit peel in pomegranates is of economic relevance,yet scarcely studied andpoorly understood in China. The aim of this study is to explore the metabolic process of the pomegranatepeel in fruit maturation period at the proteomic level.【Methods】We compared the proteomic differencesbetween‘Zhongnonghong'( red peel)and‘Sanbai'( white peel)pomegranates by two- dimensionalelectrophoresis(2-DE)and MALDI-TOF-TOF MS.【Results】892 and 890 protein spots were detectedon 2-DE gels respectively in two varieties,and 36 protein spots existed two times difference in expres-sion(P<0.05)between two varities. Compared with‘Zhongnonghong',‘Sanba'possessed 13 up-regu-lated and 19 down-regulated proteins. We identified and annotated 4 of these by using MALDI-TOF-TOF MS i.e. Cysteine synthase isoform 1(sport 1402),Cytochrome b6-f complex iron(sport 0013),pu-tative chlorophyll a/b-binding protein(sport 5108)and fructokinase-2-like(sport 0503).【Conclusion】Cysteine synthase isoform 1(sport 1402)might play a role in improving anti- oxidation capability of pomegranate in the period of fruit maturation. The other three proteins may be related to fruit peel colorchanges. The results may provide a foundation for proteomic study of pomegranate fruit development andimproving the quality of pomegranate fruit.

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