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当前位置: 首页 > 文章 > 杀鲑气单胞菌杀藻作用的初步研究 上海海洋大学学报 2020 (3) 439-446
Position: Home > Articles > Preliminary study on algaecide function of chitinase from Aeromonas salmonicida Journal of Shanghai Ocean University 2020 (3) 439-446

杀鲑气单胞菌杀藻作用的初步研究

作  者：
戴佳傲;韦芬;鲍宝龙;黄旭雄;胡鲲;龚小玲
单  位：
关键词：
杀鲑气单胞菌;几丁质酶;基因敲除;角毛藻;几丁质
摘  要：
为了研究几丁质酶对硅藻杀灭作用,以杀鲑气单胞菌(Aeromonas salmonicida)和其几丁质酶敲除菌为研究对象对牟氏角毛藻(Chaetoceros muelleri)进行共培养并探究其作用差异。结果表明:杀鲑气单胞菌野生株和缺失株分别与牟氏角毛藻共培养48 h后,野生株对牟氏角毛藻的杀藻率达到9.18%,而缺失株无明显杀藻活性;通过扫描电镜进一步发现野生株细菌能够裂解牟氏角毛藻的细胞壁,而缺失株不能。结果证实杀鲑气单胞菌因具有几丁质酶而对藻类具有杀灭作用。
译  名：
Preliminary study on algaecide function of chitinase from Aeromonas salmonicida
作  者：
DAI Jiaao;WEI Fen;BAO Baolong;HUANG Xuxiong;HU Kun;GONG Xiaoling;National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University;The Key Laboratory of Exploration and Unilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University;Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding,Shanghai Ocean University;
关键词：
Aeromonas salmonicida;;chitinase;;knockout;;Chaetoceros muelleri;;chitin
摘  要：
In order to study the functional role of chitinase in the infection of diatoms by Aeromonas salmonidae,Aeromonas salmonicida and its knockout strain were uesd to be co-culture with diatoms and explore the difference of its function.The results showed that: after 48 hours of co-cultivation of Chaetoceros muelleri with wild strains and knockout strains of Aeromonas salmonicida, the wild strains had a killing rate of 9.18% against Chaetoceros muelleri, while the knockout strains had no significant algae killing Activity; by scanning electron microscopy, it was further found that wild strains of bacteria could lyse the cell wall of Chaetoceros muelleri, but the knockout strains could not. The results confirmed that Aeromonas salmonicida has a killing effect on algae due to its chitinase.

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