作 者：
刘生杰;余为一;朱茂英;刘成文;聂传朋;李东伟
单 位：
阜阳师范学院生物系;阜阳市畜牧局;安徽农业大学生命科学学院
摘 要：
目的:提纯猪血清IgG,并分析DEAE-52层析对IgG得率的影响。方法:用硫酸铵盐析和DEAE-52阴离子交换层析两步法提纯猪血清IgG,以SDS-PAGE、Bradford法浓度测定和免疫双扩散来分析DEAE-52层析对IgG得率的影响。结果:DEAE-52层析获得两个分开明显无重叠洗脱峰,两峰均含有IgG,其中第一峰蛋白纯度达95.7%,得率为3.0～4.0mg/ml血清;第二峰IgG纯度59%。结论:DEAE-52层析能使猪血清IgG在两个洗脱峰中分布,两步法能从层析第一峰获得电泳纯IgG,对层析第二峰进一步纯化能大幅提高IgG得率。
译 名：
Effects of DEAE-52 Anion-exchanging Chromatography on Yield Rate of IgG Purified from Swine Serum
作 者：
LIU Sheng-jie1,YU Wei-yi2,*,Zhu Mao-ying1,LIU Cheng-wen3,NIE Chuan-peng1,LI Dong-wei1 (1.Department of Biology, Fuyang Teachers College, Fuyang 236041, China; 2.School of Life Science, Anhui Agricultural University, Hefei 231072, China; 3.Husbandry Bureau of Fuyang, Fuyang 236015, China)
关键词：
porcine immunoglobulin G(IgG);purify;chromatography;yield;effect
摘 要：
Objectives: To purify immunoglobulin G (IgG) from swine serum and analyze the effects of DEAE-52 chromatog- raphy on the yield rate of IgG. Methods: Porcine IgG was separated and purified from swine serum by two-step methods, SDS- PAGE and Bradfold assaying kit with saturated ammonium sulphate (SAS) precipitation followed by DEAE-52 cellulose anion- exchanging chromatography. The effects of DEAE-52 chromatography on the yield rate of IgG were defermined by SDS-PAGE and Bradford assaying kit with double immunodiffusion(DI). Results:Two different eluate peaks are obtained in DEAE-52 chromatography. IgG is also found in different eluate peaks of chromatogram by SDS-PAGE and DI.The yield rate and purity of IgG in first eluate peak are 3.0～4.0 mg/ml serum and 95.7% respectively. But the purity in second eluate peak is lower. Conclusion: DEAE-52 chromatography leads the porcine IgG to distribute in two different eluate perks. The IgG of electro- phoretic purity can be purified from the first eluate perk of DEAE-52 chromatogram by two-step methods. The yield rate of IgG can be largely increased by IgG and further purified from the second eluate perk .
