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Position: Home > Articles > The Analysis of Isozymes in Hucho taimen Chinese Journal of Fisheries 2010,23 (2) 50-54

哲罗鱼(Hucho taimen)同工酶研究

作  者:
孙源;孙中武;尹洪滨
单  位:
中国水产科学研究院黑龙江水产研究所;东北林业大学
关键词:
哲罗鱼;同工酶;组织特异性;遗传多样性
摘  要:
采用聚丙烯酰胺凝胶垂直板电泳技术对乌苏里江哲罗鱼肌肉、心脏、肝脏和眼4种组织中ADH、EST、G-6-PDH、IDH、LDH、MDH、ME、POD和SDH等9种同工酶的分布及表达进行研究。结果表明,9种酶在4种组织中的表达呈现出明显的组织特异性。遗传多样性分析共记录11个基因座位,其中有2个位点(Adh-2和Est-1位点)为多态,多态座位比例为18.2%;群体平均杂合度(H)为0.0395,位点有效等位基因数(Ne)为1.0511,Shannon’s信息指数(I)为0.068,表明哲罗鱼群体的遗传多样性程度处于较低水平;卡方检验结果表明哲罗鱼偏离Hardy-Weinberg遗传平衡。
译  名:
The Analysis of Isozymes in Hucho taimen
作  者:
SUN Yuan1,2,SUN Zhong-Wu1,YIN Hong-bin2 (1.Northeast Forestry University,Harbin 150040,China;2.Heilongjiang River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Harbin 150070,China)
关键词:
Hucho taimen;tissue-specific;genetic diversity;Isozymes
摘  要:
In this study,the distribution and expression of nine isozymes(ADH,EST,G-6-PDH,IDH,LDH,MDH,ME,POD,SDH)in four kinds of tissues(muscle,heart,liver,eyes)of Hucho taimen were analyzed using the vertical plate polyacrylamide gel electrophoresis(PAGE).The results showed that the expression of 9 isozymes in four tissues exhibited apparent tissue-specific.Genetic variation analysis recorded a total of 11 genetic loci,of which two loci(Adh-2 and Est-1 point)were polymorphic,the polymorphic proportion was 18.2%.The heterozygosity(H)was 0.0395,the effective number of alleles(Ne)per each locus was 1.0511,Shannon's information index(I)was 0.068,indicating the genetic diversity of Hucho taimen in Wusuli River was in a lower level.Moreover,chi-square test showed that Hucho taimen collected in this study deviated from Hardy-Weinberg genetic equilibrium.

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