当前位置: 首页 > 文章 > 柑橘溃疡病拮抗放线菌筛选及其抑菌机理研究 安徽农业科学 2014 (30) 10547-10549+10589
Position: Home > Articles > Screening of Antagonistic Actinomycetes spp. against Xanthomonas axonopodis pv. citri and Their Antibacterial Mechanism Journal of Anhui Agricultural Sciences 2014 (30) 10547-10549+10589

柑橘溃疡病拮抗放线菌筛选及其抑菌机理研究

作  者:
马冠华;杨镜祯;易龙;陈国康;董国菊
单  位:
赣南师范学院生命与环境科学学院;西南大学植物保护学院
关键词:
柑橘溃疡病菌;放线菌;拮抗作用;抑菌机理
摘  要:
[目的]明确保存的放线菌资源对柑橘溃疡病菌的抑制作用和抑菌机理。[方法]通过平板对峙培养法筛选对柑橘溃疡病菌(Xanthomonas axonopodis pv.citri)有抑制作用的放线菌菌株,并采用电导率法、考马斯亮蓝法和斐林试剂法测定拮抗菌株发酵滤液对溃疡病菌的影响。[结果]筛选出3个对柑橘溃疡病菌有拮抗作用的放线菌菌株,抑菌圈直径达42.0~60.0 mm,且该3株放线菌发酵液能提高溃疡病菌菌液电导率,增加还原糖和蛋白质含量。[结论]3株拮抗菌能破坏柑橘溃疡病菌细胞膜完整性,增加细胞膜通透性,引起细胞质外渗。
译  名:
Screening of Antagonistic Actinomycetes spp. against Xanthomonas axonopodis pv. citri and Their Antibacterial Mechanism
作  者:
MA Guan-hua;YANG Jing-zhen;YI Long;College of Plant Protection,Southwest University;College of Life and Environmental Sciences,Gannan Normal University;
关键词:
Xanthomonas axonopodis pv.citri;;Actinomycetes strains;;Inhibition effect;;Antibacterial mechanism
摘  要:
[Objective] The aim was to clear the inhibition and mechanism of Actinomycetes strains against Xanthomonas axonopodis pv. citri in vitro. [Method] The inhibitory ability of Actinomycetes strains against Xanthomonas axonopodis pv. citr was investigated with dual-cultured. The effect of Actinomycetesfermentation filtrate to Xanthomonas axonopodis pv. citri was measured by conductivity method,coomassie brilliant blue method and Fiji Lin reagent method. [Result]There are three antagonistic Actinomycetes strains were screened,the bacteriostatic circle diameter was from 42. 0 mm to 60. 0 mm. The sugar content,protein content and electric conductivity of X. axonopodis pv. citri were increased. [Conclusion]The fermentation filtrate of three Actinomycetes strains could destroy the cell membrane of Xanthomonas axonopodis pv. citri and lead to the leakage of cytoplasm.

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