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当前位置: 首页 > 文章 > 千岛湖细鳞鲴种群线粒体COⅠ基因变异及遗传分化研究 湖南农业科学 2011,2011 (15) 150-153
Position: Home > Articles > Gene Mutation and Genetic Differentiation of Mitochondrial COI in Xenocypris microlepis in Qiandaohu Lake Hunan Agricultural Sciences 2011,2011 (15) 150-153

千岛湖细鳞鲴种群线粒体COⅠ基因变异及遗传分化研究

作  者：
曲宪成;张勇;刘其根;张开岳;蒋骄云;冯龙
单  位：
上海海洋大学水产与生命学院
关键词：
细鳞鲴;线粒体COⅠ基因;遗传结构
摘  要：
首先利用鱼类线粒体COⅠ基因两端的保守序列设计简并引物,扩增测序获得了细鳞鲴COⅠ基因1 551 bp序列全长。然后利用COⅠ基因部分序列标记对千岛湖汾口(FK)、姜家镇(JJZ)、富文(FW)以及临岐(LQ)4个细鳞鲴群体84个样本的遗传多样性和遗传结构进行了分析,结果表明:在4个群体中共检测到4种不同单倍型。四群体的单倍型多样性(h)分别为0.500 0、0、0.142 5和0.128 7,核苷酸多样性(π)分别为0.001 9、0、0.000 2和0.000 2,其中FK群体的单倍型多样性(h)和核苷酸多样性(π)水平最高,另外3个群体单倍型多样性(h)和核苷酸多样性(π)水平都较低。AMOVA分析显示,群体间遗传变异占总变异的23.56%,群体内的遗传变异占总变异的76.44%,表明群体内变异是总变异的主要来源。研究结果表明千岛湖4个群体间没有明显的遗传分化。
译  名：
Gene Mutation and Genetic Differentiation of Mitochondrial COI in Xenocypris microlepis in Qiandaohu Lake
作  者：
QU Xian-cheng,ZHANG Yong,LIU Qi-gen,ZHANG Kai-yue,JIANG Jiao-yun,FENG Long(College of Fisheries and Life Sciences,Shanghai Ocean University,Shanghai 201306,PRC)
关键词：
Xenocypris microlepis;mitochondrial COI gene;genetic structure
摘  要：
At first,using the conserved sequence in both terminal of mitochondrial COⅠ gene in fishes to design degenerate primers,then the 1 551 bp of mitochondrial COI gene sequence of Xenocypris microlepis was obtained by amplification and sequencing,and then the genetic diversity and genetic structure of 84 samples from four Xenocypris microlepis populations(Fenkou,Jiangjiazhen,Fuwen,Linqi) in Qiandaohu Lake were analyzed by using partial mitochondrial COI gene sequence marker.The results showed that four different haplotypes were detected from four populations,theirs haplotype diversities(h) were 0.500 0,0,0.142 5 and 0.128 7 respectively,nucleotide diversities(π) were 0.001 9,0,0.000 2 and 0.000 2 respectively;among four populations of Xenocypris microlepis,Fenkou population had the highest h and π,and the others all had low h and π.The results of AMOVA analysis indicated that the genetic mutation between populations account for 23.56% of total mutation,and the genetic mutation within population account for 76.44 % of total mutation,so it proved the genetic mutation within population is the main source of total mutation.This study suggested that there were no obvious genetic differentiations among four populations of Xenocypris microlepis in Qiandaohu Lake.

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