单 位：
中国农业科学院油料作物研究所基因组学与分子生物学研究室/农业部油料作物生物学重点开放实验室
关键词：
Cre/loxP系统;位点特异性重组;转基因;载体构建
摘 要：
Cre/loxP位点特异性重组系统自发现以来,在植物基因重组领域得到了广泛应用,已成为提高转基因植物安全性的有效方法。本文介绍了该系统的基本概况及其在转基因植物表达载体构建方面的应用,尤其是在基因删除、定点整合、多基因表达以及杂种优势利用等方面的应用做了重点阐述。
译 名：
Progress in the Application Study of Cre/loxP Site-Specific Recombination System in Plants
作 者：
SUN Jia-li,YAN Xiao-hong,WANG Li-jun,WEI Wen-hui (Department of Genomics and Molecular Biology,Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences/Key Laboratory of Oil Crop Biology of the Ministry of Agriculture,Wuhan 430062)
关键词：
Cre/loxP system; site-specific recombination; transgene; construction of vector
摘 要：
The Cre/loxP site-specific recombination system has been widely applied to gene recombination in plants,and it has become a powerful tool to enhance the safety of transgenic plants. The present review describes an overview of the basic profile of Cre/loxP system and its applications in the construction of plant expression vector,especially in gene deletion,site-specific gene integration,multi-gene expression and heterosis application.
