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Position: Home > Articles > Screening and Identify of the Baicalin Microbial Transformation and Optimization of Fermentation Conditions for Baicalein by Microorganism Hubei Agricultural Sciences 2017,56 (8) 1483-1488

黄芩苷微生物转化菌的筛选鉴定及发酵条件优化

作  者:
苏龙;梁广波;龙文英;张小晴;陈夏露;黄燕燕
单  位:
玉林师范学院生物与制药学院
关键词:
黄芩苷;微生物转化;罗尔夫青霉(Penicillium rolfsii);黄芩素;优化
摘  要:
通过罗尔夫青霉(Penicillium rolfsii)发酵培养基和发酵条件优化,来提升黄芩苷转化率。采用单因素方法对氮源、发酵接种量、初始pH发酵时间、发酵温度等因素进行考查,在此基础上进行L_(16)(4~5)正交试验优化。得到的优化发酵条件是黄芩粉20 g/L,(NH_4)_2SO_4 0.6 g/L,KH_2PO_4 0.2 g/L,醋酸钠0.1 g/L,维生素C 0.1 g/L,MgSO_4 0.1 g/L,pH 5.0,料液比为1∶30,接种量10%,装液量80 mL/250 mL,发酵温度35℃,发酵时间6 d,转速180 r/min。在最优条件下,黄芩素含量达到1.83 mg/mL。
译  名:
Screening and Identify of the Baicalin Microbial Transformation and Optimization of Fermentation Conditions for Baicalein by Microorganism
作  者:
SU Long;LIANG Guang-bo;LONG Wen-ying;ZHANG Xiao-qing;CHEN Xia-lu;HUANG Yan-yan;College of Biology & Pharmacy of Yulin Normal University;
关键词:
baicalin;;microbial transformation;;Penicillium rolfsii;;baicalein;;optimize
摘  要:
The Penicillium rolfsii fermentation medium and conditions were optimized to increase the baicalin yield.Firstly,using single-factor test,the effects of these 5 parameters,i.e.,nitrogen source,the inoculation amount,initial pH of medium,fermentation time,fermentation temperature on the baicalin yield were studied.Then the key parameters of fermentation conditions were statistically optimized through orthogonal array experiments with 5 factors at 4 levels.The optimal conditions were Scutellaria baicalensis Georgi.power 20 g/L,(NH_4)_2SO_4 0.6 g/L,KH_2PO_4 0.2 g/L,sodium salt 0.1 g/L,MgSO_4 0.1 g/L,vitamin C 0.1 g/L,initial pH 5.0,solid liquid ratio 1∶30,rotation speed 180 r/min,10% inoculum size in 80 mL working volume(in250 mL flasks) at 35 ℃ for 6 d.Using these conditions,the baicalein yield was 1.83 mg/mL.

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