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当前位置: 首页 > 文章 > 斜纹夜蛾精氨酸激酶基因的克隆及mRNA表达分析 江西农业学报 2020 (5) 1-6
Position: Home > Articles > Cloning and mRNA Expression Analysis of Arginine Kinase in Spodoptera litura Acta Agriculturae Jiangxi 2020 (5) 1-6

斜纹夜蛾精氨酸激酶基因的克隆及mRNA表达分析

作  者：
孙杨;陈琼;胡妍月;秦文婧;黄水金
单  位：
关键词：
斜纹夜蛾;精氨酸激酶;克隆;mRNA表达水平
摘  要：
利用RT-PCR和RACE技术获得了斜纹夜蛾精氨酸激酶基因的全长cDNA,命名为SlAK,其GenBank登录号为HQ840714。序列分析结果表明:该cDNA全长1373 bp,其中5′和3′UTR的长度分别为65和240 bp;其开放阅读框位于66～1133 bp,编码355个氨基酸。同源性分析结果显示,精氨酸激酶基因蛋白序列享有较高的同源性,该序列具有精氨酸激酶典型的酶活性部位氨基酸序列CPTNLGT、酶活性位点氨基酸和能形成离子耦合结构的氨基酸。SlAK基因在斜纹夜蛾幼虫的头部、中肠、脂肪体和体壁内均有表达,以在中肠内的表达水平最高;SlAK基因在斜纹夜蛾幼虫不同发育期的表达量不同,mRNA表达水平在3龄达到最高峰。
译  名：
Cloning and mRNA Expression Analysis of Arginine Kinase in Spodoptera litura
作  者：
SUN Yang;CHEN Qiong;HU Yan-yue;QIN Wen-jing;HUANG Shui-jin;Plant Protection Institute, Jiangxi Academy of Agricultural Sciences;Land Resources Survey and Planning Institute of Jiangxi Department of Natural Resources;Institute of Soil and Fertilizer & Resources and Environment, Jiangxi Academy of Agricultural Sciences;
关键词：
Spodoptera litura(Fab.);;Arginine kinase;;Cloning;;mRNA expression
摘  要：
The full-length cDNA of arginine kinase gene of S. litura was obtained by reverse transcription-polymerase chain reaction(RT-PCR) and rapid amplification of cDNA ends(RACE) technology. Sequence analysis showed that the cDNA was 1373 bp in length with 65 bp in 5′ UTR and 240 bp in 3′ UTR. The open reading frame was from 66 bp to 1133 bp, encoding 355 amino acids. Homology analysis showed the protein sequence of SlAK had typical amino acid sequence CPTNLGT. The "CPTNLGT" was the active site of arginine kinase which could form the ion coupling structure. The RT-qPCR analysis showed that the mRNA expression level of SlAK was the highest in midgut though SlAK expressed also in head, fat body and cuticle of larva. SlAK gene expressed differently from the 1 st to 6 th instar and expressed highest in the 3 nd instar.

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