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Position: Home > Articles > Molecular Mapping of Phosphorus-Efficient Gene in Rice Molecular Plant Breeding 2012,10 (6) 9-15

水稻磷高效基因的初步定位

作  者：
易俊良;周德贵;卢德城;吴耀荣;李宏;黄道强;赖穗春;王重荣;王志东
单  位：
中国科学院遗传与发育生物学研究所;湖南农业大学水稻科学研究所;广东省农业科学院水稻科学研究所
关键词：
水稻;磷高效利用;近等基因导入系;基因定位
摘  要：
磷是植物生长发育所必需的大量营养元素。植物磷营养高效利用通常与根的形态、根分泌物、膜与体内磷转运以及菌根等因素有关,表现为受多基因控制。本文通过前期筛选工作,获得1份耐低磷株系。利用230对SSR和InDel引物,对低磷材料所在的回交导入系群体进行了初步定位,定位结果显示,与磷利用效率相关的基因座位有两个OsPe5和OsPe7,分别位于第5染色体的InDel520与InDel529标记之间,与InDel525标记共分离,物理距离约为900kb,和第7染色体InDel703与InDel717标记之间,与InDel713标记共分离,物理距离约为1400kb。
译  名：
Molecular Mapping of Phosphorus-Efficient Gene in Rice
作  者：
Yi Junliang 1,2* Zhou Degui 2* Lu Decheng 2 Wu Yaorong 3 Li Hong 2 Huang Daoqiang 2 Lai Suichun 2 Wang Chongrong 2 Wang Zhidong 2 Chen Liyun 1 Xie Qi 3** Zhou Shaochuan 1,2** 1 Rice Research Institute,Hunan Agricultural University,Changsha,410128;2 Rice Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou,510640;3 Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing,100101
关键词：
Rice,High-efficient phosphorus utilization,Near-isogene introgression lines,Gene mapping
摘  要：
Phosphorus(P) was an essential macronutrient for plant growth and development.High-efficient utilization of P-nutrition in plant was closely related to many factors,such as root morphology,root exudates,P-transportors across plasmalemma,P-translocation and symbiosis with microorganisms and so on.These nutritional properties were genetically controlled by multi-genes and characterized by quantitative trait loci(QTLs).In this study,a line with tolerance to low P was selected based on the identification of previous work.Backcross introgression lines(BIL) population with low-phosphorus materials were used for preliminary mapping by using 230 pairs of SSR and InDel primers.The results showed that two loci were associated with P utilization efficiency and mapped between the markers of InDel520 and InDel529 on the chromosome 5 and InDel703 and InDel717 on the chromosome 7,respectively.The locus on the chromosome 5 was co-segregated with the InDel525,about 900 kb physical distance,wher eas the locus on the chromosome 7 was co-segregated with the InDel713,approximately 1 400 kb physical distance.

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