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当前位置: 首页 > 文章 > 甘草种子水浸液对其种子萌发及GuSQS1和GubAS基因表达的影响 核农学报 2016,30 (1) 28-34
Position: Home > Articles > Effect of Aqueous Extracts From Glycyrrhiza uralensis Fisch. Seeds on Its Seed Germination and Genes Relative Expression of GuSQS1 and GubAS Journal of Nuclear Agricultural Sciences 2016,30 (1) 28-34

甘草种子水浸液对其种子萌发及GuSQS1和GubAS基因表达的影响

作  者：
张豆豆;梁新华;王俊
单  位：
宁夏大学生命科学院
关键词：
甘草种子;水浸液;萌发;基因表达;实时荧光PCR;化感;自毒作用
摘  要：
为探讨甘草种子是否存在化感自毒作用,以甘草种子为试验材料,采用常规萌发试验和实时荧光定量PCR方法,研究10g·L~(-1)浓度的甘草种子水浸液对其种子萌发率、Gu SQS1基因和Gub AS基因表达的影响。结果表明:甘草种子具有较强的化感自毒作用。10g·L~(-1)甘草种子水浸液处理下甘草种子的萌发率为62%,极显著低于对照处理(76%);该处理极显著抑制了Gub AS基因的表达,但对Gu SQS1基因的表达却具有显著促进作用。该研究结果可为人工调控栽培甘草质量提供一定参考。
译  名：
Effect of Aqueous Extracts From Glycyrrhiza uralensis Fisch. Seeds on Its Seed Germination and Genes Relative Expression of GuSQS1 and GubAS
作  者：
ZHANG Doudou;LIANG Xinhua;WANG Jun;College of Biological Science,Ningxia University;
关键词：
Glycyrrhiza uralensis Fisch;;aqueous extracts;;germination;;gene expression;;real-time PCR;;allelopathy;;auto toxictity
摘  要：
To characterize the autointoxication potential of Glycyrrhiza uralensis Fisch.,the influences of Aqueous extract from seeds of G. uralensis at a concentration of 10g·L~(-1)on germination and relative expression of Gu SQS1 and Gub AS were studied. The results indicated that the germination rate of G. uralensis seeds in the aqueous extract was 62%,which is significantly lower than the germination rate of 76% in the pure water treatment. The aqueous extract treatment also significantly inhibited the Gub AS gene expression. In contrast,the aqueous extract promoted the relative expression of the Gu SQS1 gene. Therefore,it was concluded that the seed aqueous extract of G. uralensis can inhibit its seed germination and have significant but distinct effects on the expression of two key genes involved in the bio-synthesis pathway of glycyrrhizic acid. The findings of high autointoxication of G. uralensis seeds should be valuable for agronomic production of the species.

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