Position: Home > Articles > Prokaryotic Expression and Purification of Metastasis Suppressor Gene KISS-1 and NM23-H1
Biotechnology Bulletin
2013,0
(3)
186-191
肿瘤转移抑制基因KISS-1和NM23-H1的原核表达与纯化
作 者：
左芳雷;冯秀娟;陈尚武
单 位：
中国农业大学食品科学与营养工程学院
关键词：
肿瘤转移抑制基因;KISS-1;NM23-H1;表达;纯化
摘 要：
从人胎盘组织和乳腺癌细胞中克隆得到肿瘤转移抑制基因KISS-1和NM23-H1,经Blast比对分析与GenBank公布序列同源性达100%。NM23-H1在大肠杆菌中成功表达,大部分为可溶性蛋白,占总蛋白50%以上,经纯化后纯度达到90%;而KISS-1需要与载体的部分序列融合才能够得到有效表达,表达产物为不可溶的包涵体蛋白,占包涵体总量的70%以上,经纯化后KISS-1蛋白可能形成多聚体结构。
译 名：
Prokaryotic Expression and Purification of Metastasis Suppressor Gene KISS-1 and NM23-H1
作 者：
Zuo Fanglei Feng Xiujuan Chen Shangwu(College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083)
关键词：
Metastasis suppressor gene KISS-1 NM23-H1 Expression Purification
摘 要：
Metastasis suppressor gene KISS-1 and NM23-H1 were cloned from human placenta and breast cancer cell,share 100% identity with that published in GenBank.NM23-H1 was successfully expressed in E.coli,the major amount of protein was soluble,accounting for more than 50% of total protein,the purified protein has 90% purity;however,KISS-1 should be fused to the sequence from expression vector then can effectively expressed,the expressed protein was insoluble inclusion body,accounting for more than 70% of total insoluble protein,after purification,KISS-1 may formed into polymeric structures.
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