当前位置: 首页 > 文章 > 高效液相色谱法测定乳粉中异构化乳糖的含量 乳业科学与技术 2016,39 (1) 11-13
Position: Home > Articles > High Performance Liquid Chromatography Determination of Lactulose in Milk Powder Journal of Dairy Science and Technology 2016,39 (1) 11-13

高效液相色谱法测定乳粉中异构化乳糖的含量

作  者:
刘丽君;李素琴;赵贞;岳虹;李翠枝;邵建波
单  位:
内蒙古伊利实业集团股份有限公司
关键词:
高效液相色谱法;异构化乳糖;乳粉
摘  要:
建立乳粉中异构化乳糖高效液相色谱的测定方法。采用盐酸沉淀蛋白,氨基色谱柱分离,流动相为80%乙腈溶液,示差检测器检测。每100 g乳粉中异构化乳糖添加量为0.3~2.0 g时,回收率97.4%~103.0%。定量检出限为0.3 g/100 g。该方法操作简便,检测准确,可用于乳粉中异构化乳糖的检测。
译  名:
High Performance Liquid Chromatography Determination of Lactulose in Milk Powder
作  者:
LIU Lijun;LI Suqin;ZHAO Zhen;YUE Hong;LI Cuizhi;SHAO Jianbo;Inner Mongolia Yili Industrial Group Co.Ltd.;
关键词:
high performance liquid chromatography(HPLC);;lactulose;;milk powder
摘  要:
A method for the determination of lactulose in milk powder by high performance liquid chromatography(HPLC) was developed. Lactulose was extracted with water. The analysis was performed on a NH2 column using 80% acetonitrile in water as the mobile phase. The recovery of lactulose spiked into milk powder in the range of 0.3–2.0 g/100 g was 97.4%–103.0%. The limit of detection was 0.3 g/100 g. The results indicated that this method is accurate, simple and suitable for the analysis of lactulose in milk powder.

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