Responses of pprI-transgenic Rape Seedlings to the ~(133)Cs or ~(88)Sr Stress

FENG De-yu;DAI Qi-lin;CUI Guang-yan;XIAN Xian-yi;ZHOU Wen-bo;WANG Jin;School of life science and Engineering,southwestUniversity of science and Technolog;Biotechnology Research Institute,Chinese Academy of Agricultural Science;

pprI gene;;Rape;;133Cs;;88Sr;;Stress response

The responses of pprI-transgenic and non-transgenic rape seedlings to the different133Cs and88Sr Single Stress were studied by pot experiments. Result showed that the uptake of133Cs or88Sr in rape seedlings increased with the increase of133Cs or88Sr concentration. The133Cs or88Sr contents of roots were more than that of the up-ground parts in transgenic and non-transgenic rape seedlings,and the88Sr content was higher than the133Cs content in the roots. But we found root uptake more133Cs or88Sr in transgenic rape than non-transgenic rape,and the133Cs or88Sr content of upground partsin transgenic rape was less than that in non-transgenic rape,which showed that the roots in transgenic rape more readily absorbed and fixed and transported133Cs or88Sr to the up-ground part than that in non-transgenic rape.With the increase of133Cs or88Sr concentration,the MDA content of transgenic and non-transgenic rape leaves were gradually increased,and the soluble protein content,POD and SOD activities were first rose and then declined,but the root activity decreased. Under the same treatment,the MDA content of transgenic rape was lower than that of nontransgenic rape,but the root activity,the content of soluble protein,POD and SOD activity of transgenic rape were higher than those of non-transgenic rape. Therefore,the above results indicated that pprI-transgenic rape has better ability to resist against133Cs or88Sr than non-transgenic rape. This study will provideces the theoretical and practical basis for further study the regulation mechanism of pprI gene and the gene response to133Cs or88Sr stress.