Yang Hui;Gui‐Rong Qu;Zhao YuanKai;Zhiqiang She

Shandong LuDu Bio-science Industry Co;Shandong LuDu Bio-science Industry C

cpv;lamp;method;visualized;lamp method;the detectio

[Objective] To develop a rapid and visualized detection method using loop-mediated isothermal amplification (LAMP), improve the detection rate of canine parvovirus (CPV) and reduce the testing cost. [Method] According to the conserved regions of the VP2 gene of CPV, six primers were designed to amplify the special DNA sequences by LAMP. In addition, the reaction conditions of LAMP were optimized, and the sensitivity, specificity, repeatability and stability were verified. [Result] The optimal reaction time of the LAMP method for CPV was 60 min. The products obtained by LAMP had high specificity without cross-reaction with other generic viruses. The sensitivity of the LAMP was 100 times higher than that of PCR. [Conclusion] The LAMP method for detecting CPV has high practical value. It has many advantages such as high specificity, high sensitivity, simple operation, low cost and rapid analysis, and it does not require special equipment. Therefore, this method is more suitable for the detection of CPV.