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Position: Home > Articles > Establishment of PCR-ELISA for Detecting Glyphosate Resistant Transgenic Soybean Journal of Northeast Agricultural University(English Edition) 2016,23 (2)

Establishment of PCR-ELISA for Detecting Glyphosate Resistant Transgenic Soybean

作  者:
Qiang Yao;Yuansong Wei;Mingming Fu;Yefeng Qiu;Hongtao Wen;Minghui Zhang;Ying Liu;Jinxia A
单  位:
College of Life Sciences Northeast Agricultural University Harbin 150030 China;Heilongjiang Institute of Education, Harbin 150080, China;Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China;College of Life Sciences Northeast Agricultural University Harbin 150030 Chin;Heilongjiang Forest Protection Research Institute, Harbin 150040, China
关键词:
pcr;transgenic soybean;elisa method;detecting;the results;mi
摘  要:
Abstract A PCR-ELISA method for detecting the glyphosate resistant transgenic soybean was established and optimized. The results showed that the key parameters of PCR-ELISA were as follows: the concentration of digoxin tag probe was 0.5 µmol · L −1 , the time of hybridization reaction was 15 min and the chromogenic reaction should last for 30 min. The sensitivity and the repeatability of our PCR-ELISA method were evaluated, and the results showed that it could be detected when the concentration of DNA template from transgenic soybean samples was 0.01% or higher, and the coefficient of variation of this method was less than 5% in our research condition. These results suggested that PCR-ELISA method establishment in this study had good repeatability and high precision for detecting the transgenic soybean samples.

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