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Position: Home > Articles > Isolation and Identification of Endophytic Bacteria and Analysis of Population Diversity in Different Potato Varieties China Vegetables 2016 (6) 41-46

马铃薯内生细菌的分离鉴定及种群多样性分析

作  者:
屈青松;彭万里;翟立明;刘晓;林榕姗
单  位:
山东农业大学生命科学学院山东农业微生物菌种资源保藏中心
关键词:
马铃薯;DGGE;内生细菌;鉴定
摘  要:
为了分离鉴定马铃薯内生细菌,对相同种植地点的3个不同品种马铃薯内生细菌的群落结构进行分析;从不同品种马铃薯中分离纯化菌株,进行基因序列分析和生理生化鉴定;取相同部位马铃薯组织进行PCR-DGGE操作,并对DGGE图谱上的条带进行差异性分析。最终分离得到9株内生细菌,鉴定1-1、1-2为枯草芽孢杆菌(Bacillus subtilissp.),1-3为类芽孢杆菌(Paenibacillussp.),1-4为韩国假单胞菌(Pseudomonas koreensissp.),1-5、1-6、2-3为解淀粉芽孢杆菌(Bacillusamyloliquefacienssp.),2-2为苍白杆菌(Ochrobactrumsp.),3-6为副球菌(Paracoccussp.)。DGGE图谱表明不同品种的马铃薯内生细菌既有差异性又有相似性。最终结果表明3个品种马铃薯内生细菌具有较丰富的种群多样性,且差异性明显,品种可能是影响马铃薯内生细菌群落结构的重要因素。
译  名:
Isolation and Identification of Endophytic Bacteria and Analysis of Population Diversity in Different Potato Varieties
作  者:
QU Qing-song;PENG Wan-li;ZHAI Li-ming;LIU Xiao;LIN Rong-shan;College of Life Science,Shandong Agricultural University,Shandong Agricultural Resource Collection of Microorganisms;
关键词:
DGGE;;Potato;;Endophytic bacteria;;Identification
摘  要:
In order to isolate and identify endophytic bacteria in potato and analyze the community structure of 3 different potato varieties,this experiment isolated and purified strains from different potato varieties,and carried out gene sequence analysis and physiological and biochemical identification.The PCR-DGGE operation was performed on the same site in potato,and analyzed the bands on DGGE.Nine strains of endophytic bacteria were isolated,identified 1-1,1-2 as Bacillus subtilis sp.,1-3 as Paenibacillus sp.,1-4 as Pseudomonas koreensis sp.,1-5、1-6、2-3 as Bacillus amyloliquefaciens sp.,2-2 as Ochrobactrum sp.,3-6 as Paracoccus sp.The DGGE bands indicated that there were both differences and similarities in different potato varieties.The results showed that 3 potato varieties had rich population diversity,and their differences were also obvious.The species may be an important factor influencing the structure of endophytic bacteria community.

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