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Position: Home > Articles > Production of transgenic pigs with phytase gene by sperm-mediated gene transfer Acta Agriculturae Shanghai 2013,29 (5) 12-15

精子载体法获得植酸酶转基因猪

作者：

吴斌;戴建军;张廷宇;张树山;吴彩凤;顾晓龙;陈会兰;张德福

单位：

上海市农业科学院畜牧兽医研究所

关键词：

精子载体法;植酸酶基因;转基因猪

摘要：

将带有植酸酶基因的重构质粒酶切纯化后,与去除精浆的猪新鲜精液按比例混合,17℃孵育处理使重构质粒结合到精子头部,通过人工授精对6头受体母猪进行配种,4头受孕,产仔38头。经PcR对特异性片段扩增,结果表明:有6头PcR结果为阳性,阳性率为15.8%。扩增片段经克隆、测序、比对,与阳性对照组序列相似率均在99%以上,说明目的基因已转入阳性个体体内。

译名：

Production of transgenic pigs with phytase gene by sperm-mediated gene transfer

作者：

WU Bin;DAI Jian-jun;ZHANG Ting-yu;ZHANG Shu-shan;WU Cai-feng;GU Xiao-long;CHEN Hui-lan;ZHANG De-fu;Animal Husbandry and Veterinary Research Institute,Shanghai Academy of Agricultural Sciences;Shanghai Key Laboratory of Agricultural Genetics and Breeding;College of Life and Basic Sciences,Sichuan Agricultural University;

关键词：

Sperm-mediated gene transfer;;Phytase gene;;Transgenic pig

摘要：

After digested and purified,the recombinant plasmid with phytase genes was mixed proportionally with the fresh seminal fluid whose seminal plasma was removed,and then incubated at17℃ to make the plasmid combine with the spermhead.The joining with 6 sows was carried out by artificial insemination,producing 4 pregnant sows and farrowing 38 piglets.PCR amplification of the specific fragments showed that the PCR products of 6 piglets were positive,and the cloning,sequencing and alignment indicated that all the rates of sequence similarity between the amplified fragments and the control group were over 99%,demonstrating that the phytase gene had been transferred into the positive piglets.

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精子载体法获得植酸酶转基因猪

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