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Position: Home > Articles > Study on the Tissue Culture and Rapid Regeneration of Northern Shaanxi Wild Plant Lilium pumilum DC. Northern Horticulture 2011 (22) 123-125

陕北野生山丹丹组培快繁技术研究

作  者:
齐向英;陈宗礼;薛皓;王延峰;刘维;杨鹏
单  位:
延安大学生命科学院陕西省区域生物资源保育与利用工程技术研究中心
关键词:
野生;山丹丹;快繁
摘  要:
以陕北地区野生的山丹丹鳞片为试材,采用单因子和正交设计研究了不同浓度IBA和NAA及其组合对山丹丹组培快繁的影响。结果表明:适合山丹丹初代培养的培养基为MS+6-BA 1.5mg/L+NAA 0.01mg/L,芽诱导率达到100%;继代培养为MS+6-BA 2.5mg/L+IBA0.2mg/L,繁殖系数达到9.8;生根培养为1/2MS+IBA 0.3mg/L,生根率达到100%。
译  名:
Study on the Tissue Culture and Rapid Regeneration of Northern Shaanxi Wild Plant Lilium pumilum DC.
作  者:
QI Xiang-ying,CHEN Zong-li,XUE Hao,WANG Yan-feng,LIU Wei,YANG Peng(College of Life Sciences,Yan'an University,Shaanxi Engineering and Technological Research Center of Conversation and Utilization of Biological Resources,Yan'an,Shaanxi 716000)
关键词:
wild;Lilium pumilum DC.;rapid propagation
摘  要:
Taking Shaanxi wild Plant Lilium pumilum DC.pellet flake material,using single factor and orthogonal design,to study the effect of different concentration of IAA,NAA on the tissue culture and rapid propagation of Lilium pumilum DC.process.The results showed that MS+6-BA 1.5 mg/L+NAA 0.01 mg/L suitable for primary culture,the bud induction rate was 100%.MS+6-BA 2.5 mg/L+IBA 0.2 mg/L was for subculture,propagation coefficient was 9.8.Rooting was 1/2MS+IBA 0.3 mg/L,rooting rate of 100%.

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