中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 金龟子部分种类COI基因序列比较分析 沈阳农业大学学报 2009,40 (6) 688-692
Position: Home > Articles > Sequence Analysis of mtDNA-COI Gene in the Partial Species of Scarabeoidea Journal of Shenyang Agricultural University 2009,40 (6) 688-692

金龟子部分种类COI基因序列比较分析

作  者：
孙娜;郭晓华;刘广纯
单  位：
沈阳大学生物与环境工程学院
关键词：
金龟总科;COI基因;序列分析
摘  要：
对金龟总科(Scarabaeoidea)6种金龟子线粒体细胞色素氧化酶I(COI)基因部分序列进行了PCR扩增和序列测定,并从GenBank中检索4个种的COI基因序列,比较其同源性并获得了长度为624bpCOI片段。10个种的COI序列分析结果表明:该基因序列共有440个变异位点,192个简约信息位点,表现出明显的A+T偏倚(63.6%)。转换(TV)和颠换(TS)在科内、科间存在明显差异。科内种间TV+TS小,各科不同种与外群之间TV+TS较大;TV/TS则呈现相反的趋势。以黑蜣科(Passalidae)的具角黑艳甲(Odontotaenius disjunctus)作为外群,采用P-distance法重建了10种金龟子邻接树(N-J)和最小进化树(ME)拓扑结构,结果表明:丽金龟属内科内、鳃金龟科内的不同种分别优先聚集,再与其他科相聚集,显示科内、属内的种间有较近的亲缘关系,说明COI基因序列可用来研究属内或科内种群的系统发育问题。
译  名：
Sequence Analysis of mtDNA-COI Gene in the Partial Species of Scarabeoidea
作  者：
SUN Na,GUO Xiao-hua*,LIU Guang-chun (College of Biological and Environmental Engineering,Shenyang University,Shenyang 110044,China)
关键词：
Scarabaeoidae;COI gene;sequence analysis
摘  要：
The mtDNA-COI gene fragments of 6 Scarabeoidea species were amplified and sequenced by PCR and the COI partial sequences of 4 relative species were retrieved from GenBank.The homologus sequences were compared and obtained partial nucleotide sequence of COI(624bp).The analysis results of 10 sequences of COI gene showed that there were 440 variable sites and 192 parsimony-informative sites in the 624 nucleotides of the data.Scarabaeoidea exhibited a 63.6%Arginine and Thymine bias.The distinct variation rate of transition and transversion among different species of intra-family and interfamily in Scarabaeoidea was determined.The numbers of TV+TS of COI sequences among different species in the same family were higher than those in the different families.The ratio of TV and TS showed the opposite trend.The topological trees of Neighbour Joining and Minimum Evolution constructed by P-distance method using Passalidae Odontotaenius disjunctus as the outgroup revealed that the species in Rutelidae and Melolonthidae were grouped together first,and showed close relationship. They were further grouped together with the species of other families.The analysis results of COI gene sequences and phylogenic threes showed that there were close relationships among species of the genus or the family.The phylogenetic relationships of the species in genera or family could be well resolved with COI gene sequences.

相似文章

导出引用
计量
文章访问数: 10
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46