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Position: Home > Articles > SDS-PAGE Analysis of Phase Change-related Proteins in Apple(Malus domestica Borhk.) Acta Horticulturae Sinica 2008 (7) 1059-1064

苹果实生树阶段转变特异蛋白质的SDS-PAGE分析

作  者：
曾广娟;李春敏;张新忠;田义;陈东玫;赵永波;董文轩;ZENG Guang-juan;LI Chun-min;ZHANG Xin-zhong;TIAN Yi;CHEN Dong-mei;ZHAO Yong-bo;DONG Wen-xuan
单  位：
沈阳农业大学园艺学院;河北省农林科学院昌黎果树研究所;中国农业大学园艺植物研究所
关键词：
苹果;阶段转变;蛋白质;SDS-PAGE
摘  要：
利用聚丙烯酰胺凝胶电泳技术(SDS-PAGE)分析了6株苹果实生树(‘红玉’ב金冠’)叶片中蛋白质随节位升高的动态变化。结果表明,随节位升高蛋白质带发生变化,共发现了3条阶段转变特异蛋白质带,分子量分别为55kD、19kD和17kD。其中55kD蛋白质表现为定性变化,有5株实生树在36~75节出现,到101~126节消失;19kD蛋白质在实生树低节位含量高,在86~100节之后含量突然降低;17kD蛋白质在实生树低节位含量低,随着植株的发育蛋白质在36~50节含量突然增加并保持稳定,到91~105节后含量突然下降。认为这3种蛋白质是苹果实生树阶段转变的特异蛋白质。
译  名：
SDS-PAGE Analysis of Phase Change-related Proteins in Apple(Malus domestica Borhk.)
作  者：
ZENG Guang-juan1,2, LI Chun-min1, ZHANG Xin-zhong3, TIAN Yi2, CHEN Dong-mei1, ZHAO Yong-bo1, and DONG Wen-xuan2 (1Changli Institute of Pomology, Hebei Academy of Agricultural and Forestry Sciences, Changli, Hebei 066600, China;2College of Horticulture, Shenyang Agricultural University, Shenyang 110161, China;3 Institute for Horticultural Plants, China Agricultural University, Beijing 100094, China)
关键词：
Malus domestica; phase change; protein; SDS-PAGE
摘  要：
Dynamic changes of proteins in leaves from different nodes in 6 hybrid seedlings (Jonathan ×Golden Delicious) were analyzed with SDS-polyacrylamide gel electrophoresis in apple. Three protein bands were found specific in different phases. The molecular mass of the 3 protein bands were approximately 55 kD, 19 kD and 17 kD. The 55 kD protein presented qualitatively between No.36-75 and No.101-126 nodes. The change of 19 kD and 17 kD proteins was found quantitatively. In leaf samples taken below nodes No. 86-100, the content of 19 kD protein was higher, but a sharp decrease occurred in leaves above nodes No.100. The content of 17 kD protein kept high between No.36-50 and No.91-105 nodes. It is therefore suggested that the 3 proteins were phase change-related in apple seedlings.

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