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当前位置: 首页 > 文章 > 反相高效液相色谱法对小鼠血清中基因重组人促肾上腺皮质激素前体及其代谢物的测定 热带生物学报 2019 (1) 83-88
Position: Home > Articles > Determination of Recombinant Human Adrenocorticotropic Hormone Precursor Protein and Its Metabolite in Mouse Serum by RP-HPLC Journal of Tropical Biology 2019 (1) 83-88

反相高效液相色谱法对小鼠血清中基因重组人促肾上腺皮质激素前体及其代谢物的测定

作  者：
武新丽;韦双双;裴业春;赵景锋;王大勇
单  位：
海南大学海南省热带生物资源可持续利用重点实验室
关键词：
基因重组;人促肾上腺皮质激素;ACTH;前体;HPLC
摘  要：
采用反相高效液相色谱(RP-HPLC)法测定分析静脉注射基因重组人促肾上腺皮质激素(ACTH)前体蛋白(ProACTH141)后小鼠血清中ProACTH141及其代谢产物ACTH(1-39)的含量变化。结果表明,ProACTH141的半衰期(T_(1/2))为21.9 min;其代谢物ACTH(1-39)在血清中的达峰时间(T_(max))为20 min。ProACTH141和代谢产生的ACTH(1-39)的药时曲线下面积(AUC)分别为538.2和88.2 (mg·L~(-1))·min~(-1)。该方法操作简便,灵敏度高,结果准确,可用于基因重组人促肾上腺皮质激素前体蛋白的药物代谢动力学分析。
译  名：
Determination of Recombinant Human Adrenocorticotropic Hormone Precursor Protein and Its Metabolite in Mouse Serum by RP-HPLC
作  者：
WU Xinli;WEI Shuangshuang;PEI Yechun;ZHAO Jingfeng;WANG Dayong;Hainan Key Laboratory of Sustainable Utilization of Tropical Bioresources, Hainan University;Laboratory of Biotechnology and Molecular Pharmacology, College of Biology, Institute of Tropical Agriculture and Forestry, Hainan University;College of Animal Sciences, Institute of Tropical Agriculture and Forestry, Hainan University;
单  位：
WU Xinli%WEI Shuangshuang%PEI Yechun%ZHAO Jingfeng%WANG Dayong%Hainan Key Laboratory of Sustainable Utilization of Tropical Bioresources, Hainan University%Laboratory of Biotechnology and Molecular Pharmacology, College of Biology, Institute of Tropical Agriculture and Forestry, Hainan University%College of Animal Sciences, Institute of Tropical Agriculture and Forestry, Hainan University
关键词：
Gene recombination;;human adrenocorticotropic hormone;;ACTH;;precursor;;HPLC
摘  要：
The concentrations of recombinant human adrenocorticotropic(ACTH) hormone precursor protein(ProACTH141) and its metabolite ACTH(1-39) in mouse blood serum were determined by using a reversed-phase high performance liquid chromatography(RP-HPLC). In the Rp-HPLC method Inertsil~® C18 column(4.6×150 mm, ∅5 μm) was adopted, and ACTH(1-24) was used as an internal standard. Linear gradient elution was performed by proportional mixing of 0.1% TFA buffer(mobile phase A) and acetonitrile containing 0.1% TFA(mobile phase B). HPLC condition: flow rate, 1 mL·min~(-1); detection wavelength, 280 nm; column temperature, room temperature. Pharmacokinetic analysis showed that the half-life(T_(1/2)) of ProACTH141 was 21.9 min and that the peak retention time(T_(max)) of its metabolite ACTH(1-39) in the serum was 20 min. The area under the curves of ProACTH141 and ACTH(1-39) was 538.2(mg·L~(-1))·min~(-1). and 88.2(mg·L~(-1))·min~(-1), respectively. The RP-HPLC method is easy to operate, and high in sensitivity and accuracy and can hence be used for pharmacokinetic analysis of the recombinant human adrenocorticotropic hormone precursor protein.

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