当前位置: 首页 > 文章 > 银中杨DREB2A基因RNA干扰载体构建及转化农杆菌的研究 吉林农业大学学报 2010,32 (3) 254-257+270
Position: Home > Articles > Construction of RNAi Vector of DREB2A Gene from Populus albaxp and Study on its Transformation into Agrobacterium Journal of Jilin Agricultural University 2010,32 (3) 254-257+270

银中杨DREB2A基因RNA干扰载体构建及转化农杆菌的研究

作  者:
王磊;迟晓娟;张闯;杨微;宋黎黎;李海燕
单  位:
吉林农业大学生物反应器与药物开发教育部工程研究中心
关键词:
RNA干扰载体;Gateway技术;杨树DREB2A基因
摘  要:
应用Gateway技术体系替代传统载体构建方法,构建了银中杨(Populus albaxp)DREB2A基因的RNA干扰载体,并将其转入根癌农杆菌EHA105中。该RNAi表达载体的构建对进一步研究杨树DREB2A转录因子基因的功能具有重要意义。
译  名:
Construction of RNAi Vector of DREB2A Gene from Populus albaxp and Study on its Transformation into Agrobacterium
作  者:
WANG Lei,CHI Xiao-juan,ZHANG Chuang,YANG Wei,SONG Li-li,LI Hai-yanMinistry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development,College of Life Science,Jilin Agricultural University,Changchun 130118,China
关键词:
vector of RNA interference;Gateway technique;DREB2A gene of Populus albaxp
摘  要:
RNA interference(RNAi)is a newly gene silencing technique used to study gene function and carry out disease therapy.In this study,RNAi vector of DREB2A gene from Populus albaxp(PaDREB2A)was constructed by using Gateway technique and was introduced into Agrobacterium tumefaciens EHA105.The construction of this RNAi vector laid an important foundation for further gene function studies of PaDREB2A.

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